Gonadotropin hyperstimulation influences the35S-methionine metabolism of mouse preimplantation embryos |
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| Authors: | Alex M. M. Wetzels Marnix T. Artz Henny J. M. Goverde Bart A. Bastiaans Carl J. C. M. Hamilton Rune Rolland |
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| Affiliation: | (1) Department of Gynecology and Obstetrics, University Hospital Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands |
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| Abstract: | The effects of gonadotropin stimulation on mouse embryo uptake and incorporation of35S-methionine were studied. We found that the uptake of35S-methionine was reduced in embryos of stimulated females in both the two-cell and the blastocyst developmental stage. The incorporation of35S-methionine into protein was not statistically significantly different between the embryos of stimulated and those of unstimulated females. Qualitatively, protein synthesis was equal in both groups as determined with one-dimensional SDS-PAGE. The results are discussed and we conclude that mouse embryo viability in vivo is decreased by ovarian stimulation. |
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| Keywords: | mouse embryos in vivo gonadotropin hyperstimulation 35S-methionine metabolism |
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