电针对WKY模型大鼠抑郁样行为及缰核c-fos蛋白的影响

目的 观察电针对WKY抑郁模型大鼠行为学及缰核c-fos蛋白表达的影响。方法 将24只雄性WKY大鼠随机分为电针组(EA)、假针组(sham EA)、模型组(model),每组8只。另将Wistar雄性大鼠8只设为正常组(normal)。通过旷场实验、糖水实验、强迫游泳实验对大鼠进行行为学评价,并用免疫荧光方法检测大鼠缰核中c-fos表达。结果 与正常组比较,模型组旷场实验的水平运动、垂直运动和蔗糖水摄入比均明显下降(P0.05),强迫游泳的不动时间和缰核c-fos表达均增加(P0.05)。与假针组比较,电针组旷场实验的水平运动和垂直运动、蔗糖水摄入比均明显增加(P0.05),强迫游泳的不动时间和缰核c-fos表达减少(P0.05)。结论 电针可改善WKY抑郁模型大鼠的行为异常,明显降低缰核中c-fos的表达,这可能是电针治疗抑郁症的机制之一。

Effects of electroacupuncture on the behavior and c-fos of habenular nucleus in the depressive-like rats with WKY model

  Objective:To observe the effects of electroacupuncture(EA) on depressive-like behavior and c-fos protein expression of habenular nucleus in the WKY rat model. Methods24 male WKY rats were randomly divided into the EA, sham and model groups,8 in each group.8 Wistar male rats were set as normal group. The behaviors were evaluated by open field test, sucrose preference test and force swimming test, the expression of c-fos was detected by immunofluorescence.  Results:Compared with the control group , the behaviors of open field test and Sucrose preference test in model group decreased significantly (P<0.05),and the immobile time of force swimming test and c-fos expression of habenular nucleus increased significantly (P<0.05). Compared with sham group , the behaviors of open field test and Sucrose preference test in EA group increased significantly (P<0.05),and the immobile time of force swimming test and c-fos expression of habenular nucleus decreased significantly (P<0.05).  Conclusion:Electroacupuncture cauld improve the depressive- like behaviors of WKY rats and decrease the expression of c-fos protein in habenular nucleus. It may be one of the mechanisms of electroacupuncture on depression.