| 针刺对慢性高眼压后兔模型NGF-TrkA及AKT的影响 |
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| 引用本文： | 陆欣玲,杨扬,俞莹,李瑞玲,熊晔,李一婧,马文,沈卫东.针刺对慢性高眼压后兔模型NGF-TrkA及AKT的影响[J].上海中医药杂志,2016,50(7):83-87. |
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| 作者姓名： | 陆欣玲 杨扬 俞莹 李瑞玲 熊晔 李一婧 马文 沈卫东 |
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| 作者单位： | 上海中医药大学门诊部,上海,201203,上海中医药大学科技实验中心,上海,201203,上海中医药大学附属曙光医院眼科,上海,201203,上海中医药大学门诊部,上海,201203,上海中医药大学门诊部,上海,201203,上海中医药大学附属曙光医院针灸科,上海,201203,上海中医药大学附属曙光医院针灸科,上海,201203,上海中医药大学附属曙光医院针灸科,上海,201203 |
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| 基金项目： | 上海市科委科研计划项目(14401971700)；上海中医药大学预算内项目(2014YSN29) |
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| 摘 要： | 目的通过观察内源性NGF-TrkA及下游通路的分子基因转录特点,探讨针刺治疗对高眼压后兔视网膜神经节细胞的保护作用。方法 30只新西兰兔,采用随机数字表法随机将8只设为正常组,剩余22只造模。再次采用随机数字表法将造模成功后16只随机分为模型组、针刺组,每组8只兔。模型组与针刺组以复方卡波姆诱导建立慢性高眼压兔模型。造模2周后,针刺组开始给予毫针"合谷(LI4)、睛明(BL1)、球后(Ex-HN07)"穴治疗,每周3次,隔天1次,共治疗4周(12次);正常组和模型组不予治疗。治疗结束后,取3组兔眼球,行视网膜HE染色观测组织内形态学变化;采用RT-PCR法检测视网膜内神经生长因子信使核糖核酸(NGF mRNA)、神经生长因子受体酪氨酸激酶信使核糖核酸(TrkA mRNA)和神经营养素受体信使核糖核酸(p75 mRNA)的转录水平;以Western blotting法检测视网膜内NGF信号通路下游分子AKT(p-AKT)的表达水平。结果在治疗过程中,针刺组眼压均值波动在20.86~22.23 mm Hg之间,模型组眼压波动在23.82~25.49 mm Hg之间;造模后模型组、针刺组各时间点眼压与正常组比较,差异均有统计学意义(P0.05)。模型组视网膜整体结构有明显变化,内核层、外核层均排列混乱且两层之间边界不清,且内核层的双极细胞和外核层的视杆细胞、视锥细胞数量有所减少,神经节细胞层显示细胞核间距增大,数量有所减少。与模型组相比,针刺组内核层双极细胞数量稳定,内核层与外核层之间边界尚清,神经节细胞层细胞核间距减小,数量有所增加。视网膜内NGF mRNA、TrkA mRNA转录水平均增强,p75 mRNA转录水平降低,下游信号分子AKT表达针刺组较正常组增强,针刺组较模型组增强。结论①针刺作用于慢性高眼压后兔模型,能稳定眼压,可以使视网膜病理损害减轻,稳定神经节细胞数量,有效保护慢性高眼压后兔模型的RGC。②针刺可以提升NGF-TrkA在慢性高眼压兔模型视网膜组织内的转录水平,降低p75在慢性高眼压兔模型视网膜组织内的转录水平,提升下游信号分子AKT的磷酸化水平。
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| 关 键 词： | NGF-TrkA p75 AKT 针刺 高眼压 视网膜神经节细胞 |
| 收稿时间： | 2016/3/7 0:00:00 |
The change of NGF-TrkA and AKT after acupuncture treatment for chronic high intraocular pressure on the rabbit model |
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| LU Xin-ling,YANG Yang,YU Ying,LI Rui-ling,XIONG Ye,LI Yi-jing,MA Wen and SHEN Wei-dong.The change of NGF-TrkA and AKT after acupuncture treatment for chronic high intraocular pressure on the rabbit model[J].Shanghai Journal of Traditional Chinese Medicine,2016,50(7):83-87. |
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| Authors: | LU Xin-ling YANG Yang YU Ying LI Rui-ling XIONG Ye LI Yi-jing MA Wen and SHEN Wei-dong |
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| Institution: | The Outpatient Department of Shanghai University of Traditional Chinese Medicine, Shanghai, 201203,The Experiment Center of Science and Technology of Shanghai University of Traditional Chinese Medicine, Shanghai, 201203,The Ophthalmology Department of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203,The Outpatient Department of Shanghai University of Traditional Chinese Medicine, Shanghai, 201203,The Outpatient Department of Shanghai University of Traditional Chinese Medicine, Shanghai, 201203,The Acupuncture Department, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203,The Acupuncture Department, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203 and The Acupuncture Department, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203 |
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| Abstract: | |
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| Keywords: | NGF-TrkA p75 AKT acupuncture high intraocular pressure retinal ganglion cells |
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