TLR4蛋白在小鼠肝脏部分缺血再灌注损伤中时序性表达及意义

目的通过研究Toll样受体4(Toll-like receptor-4,TLR4)蛋白在小鼠肝脏部分缺血再灌注损伤模型中的动态表达,探讨TLR4受体的激活与肝功能损伤间的关系.方法以BALB/c小鼠复制肝脏部分缺血再灌注损伤模型,采用免疫组织化学染色方法动态观察TLR4在肝脏的分布,并用HPIAS-1000图文报告系统分析结果,同时动态监测血浆谷丙转氨酶水平及门静脉血清内毒素水平.结果肝脏左中叶缺血1 h后,不同的再灌注时间,缺血肝叶内有增强的TLR4蛋白的阳性表达,以再灌注1 h的表达最为强烈,阳性细胞主要是Kupffer细胞及血管内粒单核细胞,且TLR4的动态表达与肝功能的损伤不平行.门静脉血清内毒素水平在各时间点与假手术组相比无显著差异(P＞0.05).结论在非内毒素血症下,TLR4蛋白在肝脏缺血再灌注损伤过程中有动态表达.TLR4的激活参与了肝脏缺血再灌注损伤的过程.

Dynamic TLR4 Potein Expression and It's Significance in Partial Hepatic Ischemia/reperfusion Injury in Mice

Objective To study the dynamic TLR4 protein expression under the condition of partial hepatic ischemia/reperfusion injury in mice and explore the relationship between the expression and liver injury.Methods Mice were used in a model of partial hepatic ischemia/reperfusion(I/R) injury, the distribution of TLR4 in liver was observed with immunohistochemical stains. And the level of plasma ALT and endotoxin in portal vein were measured.Results After restoration of blood supply, the expression of TLR4 protein were strengthened in different time point.The most intensive expression was present at 1 hour reperfusion. Kupffer cells were the most positively expressed cell type. Granno-monocyte and liver sinus epithelial cell were the second. There were no positive reaction in hepatocytes. The expression of TLR4 protein didn't parellel with liver injury. And no endotoxemia appeared during the whole process(P>0.05).Conclusion Under the condition of non-endotoxemia in the model of partial hepatic ische mia/reperfusion injury, there were strengthened expression of TLR4 protein in some cell types in liver. The activation of TLR4 protein may play roles in the pathophysiological process of ischemia/reperfusion injury.