| 转染金属硫蛋白基因1A促进大鼠肝星状细胞增殖 |
| |
| 引用本文： | 王科,陈平圣. 转染金属硫蛋白基因1A促进大鼠肝星状细胞增殖[J]. 东南大学学报(医学版), 2009, 28(3): 189-193 |
| |
| 作者姓名： | 王科 陈平圣 |
| |
| 作者单位： | 东南大学基础医学院,病理学与病理生理学系,江苏,南京,210009 |
| |
| 摘 要： | 目的: 观察金属硫蛋白基因1A(MT1A)对肝星状细胞(HSCs)增殖的影响.方法: 构建MT1A基因真核表达质粒,转染HSCs.通过RT-PCR、Western blotting法检测导入的质粒在HSCs中的表达,采用AgNOR染色法及Ki-67抗原免疫细胞化学染色检测 HSCs增殖指数.结果: MT1A基因在HSCs中过表达;转染MT1A基因组每个细胞AgNOR颗粒数(5.43±0.45)较pcDNA3.1组(3.94±0.39)及非转染组(4.14±0.34)均高(P<0.05);免疫细胞化学染色结果显示,转染MT1A基因组细胞Ki-67染色呈阳性,而对照组为阴性.结论: 外源MT1A基因在HSCs中过表达可促进其增殖.
|
| 关 键 词： | 肝星状细胞 金属硫蛋白 增殖 肝纤维化 大鼠 |
Metallothionein gene transfected enhancing the proliferation of hepatic stellate cells |
| |
| WANG Ke,CHEN Ping-sheng. Metallothionein gene transfected enhancing the proliferation of hepatic stellate cells[J]. Journal of Southeast Univ: Medical Sci Ed, 2009, 28(3): 189-193 |
| |
| Authors: | WANG Ke CHEN Ping-sheng |
| |
| Affiliation: | Department of Pathology and Pathophysiology;School of Basic Medical Science;Southeast University;Nanjing 210009;China |
| |
| Abstract: | Objective To investigate the effect of metallothionein 1A gene(MT1A) on the proliferation of rat hepatic stellate cells(HSCs) in vitro.Methods MT1A expressing plasmid(MT1A-pcDNA3.1) and pcDNA3.1 empty plasmid were transfected into HSCs by fuGENE-6 transfection reagent respectively.The expression of MT1A in HSCs was detected by RT-PCR and Western blotting,and the HSCs proliferation changes were assessed by AgNOR staining and immunocytochemistry staining of the Ki-67 antigen.Results It was found that MT1A mRN... |
| |
| Keywords: | hepatic stellate cells metallothionein proliferation hepatic fibrosis rats |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
