In vivo release of neurotransmitters in the medial basal hypothalamus of the monkey |
| |
Authors: | G. Roosen-Runge M. Epler E. Düker E. Fuchs R. A. Siegel J. Demling Dr. W. Wuttke |
| |
Affiliation: | (1) Abteilung für Reproduktionsbiologie, Deutsches Primatenzentrum, D-3400 Göttingen, FRG;(2) Abteilung für Neurobiologie, Max-Planck-Institut für Biophysikalische Chemie, D-3400 Göttingen, FRG |
| |
Abstract: | Summary In vivo release rates of norepinephrine (NE), epinephrine (E), dopamine (DA), gammaaminobutyric acid (GABA), glutamate (GLU) and beta-endorphin ( E) in the medial basal hypothalamus (MBH) of unanaesthetized female macaca fascicularis monkey, and the effects thereon of estrogen (E2) treatment, have been estimated using pushpull perfusion methodology. DA, NE, E, GABA, GLU and E were all detectable in 30 min perfusate fractions. No direct correlation between their release rates and those of LH and PRL could be observed. E2 induced an initial decrease, then an increase, in LH and PRL secretion, and concomitant changes in the release patterns of DA, NE, E. GABA and GLU were apparent. This study demonstrates that in vivo push-pull perfusion methodology may be applied to the unanaesthetized monkey, and when combined with venous catheterization for serial blood sampling may prove to be a powerful tool in the investigation of the central molecular events governing neuroendocrine functions. |
| |
Keywords: | Push-pull cannula Hypothalamus Neurotransmitters LH Prolactin |
本文献已被 SpringerLink 等数据库收录! |
|