肝癌患者组织中CyclinE、p27kip1和Ki-67蛋白的表达与临床特征的相关性

目的 分析肝癌患者组织细胞周期素E(Cyclin E)、抑制因子p27kip1和核抗原-67(Ki-67)基因和蛋白的表达及与临床特征的关系.方法 选择肝癌患者共90例(观察组),同期选择50例肝脏血管瘤患者(对照组),采用免疫组化染色法检测观察组肿瘤组织和癌旁正常组织与对照组的Cyclin E、p27kip1和Ki-...

Expressions of Cyclin E,p27kip1 and Ki-67 proteins in liver cancer tissues and their relationship with clinical characteristics

Objective To analyze expressions of Cyclin E,inhibitor p27kip1 and nuclear antigen-67 genes(Ki-67)and proteins in liver cancer tissues and their relationship to clinical characteristics.Methods Totally 90 patients with hepatocellular carcinoma(observation group)and 50 patients with hepatic hemangioma(control group)were selected.The Cyclin E,p27kip1 and Ki-67 proteins were detected by immunohistochemical staining in tumor tissues and adjacent tissues of observation group and control group.The expressions of Cyclin E,p27kip1 and Ki-67 mRNAs were detected by RT-PCR,and the relationships between expressions of Cyclin E,p27kip1,Ki-67 and patients’clinical features were analyzed.Results The positive expression rates and mRNAs levels of Cyclin E and Ki-67 of tumor tissues in observation group were significantly higher than adjacent tissues in observation group and control group,while positive expression rate and mRNA level of p27kip1 of tumor tissues were lower than adjacent tissues in observation group and control group(χ2=33.91,23.77,36.44,23.17,-16.36,-7.81,t=20.40,17.47,11.26,9.69,-14.16,-14.84,P<0.05).The positive expressions of Cyclin E,Ki-67 andp 27kip1 proteins of tumor tissues in observation group were not related to patients’sex,age and tumor diameter(χ2=0.64,0.18,0.51,0.49,0.17,0.62,0.02,1.44,0.86,P>0.05),while were close related to TNM staging,blood or lymphatic metastasis,and differentiation of tumors(χ2=19.00,7.32,10.40,10.79,7.79,9.37,15.26,6.58,6.67,P<0.05).Conclusion The occurrence and development of hepatocellular carcinoma are closely related to abnormal expressions of tissues Cyclin E,p27kip1 and Ki-67 proteins.