| HPLC法测定番石榴叶、苦瓜干和苦丁茶中槲皮素的含量 |
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| 引用本文： | 邓亚东,严志红,周旭丹. HPLC法测定番石榴叶、苦瓜干和苦丁茶中槲皮素的含量[J]. 中国航天工业医药, 2011, 0(3): 31-32 |
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| 作者姓名： | 邓亚东 严志红 周旭丹 |
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| 作者单位： | [1]广州医学院附属第三医院,510150 [2]广东药学院药科学院,510150 |
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| 摘 要： | 目的建立测定番石榴叶、苦瓜干和苦丁茶中槲皮素含量方法。方法采用DiamonsiLTM C18（250mm×4.6mm,5μm）,以甲醇-0.5%磷酸溶液（58：42）为流动相,检测波长为372nm,流速：1.0ml/min。结果槲皮素在1.064～10.64μg/ml之间成良好线性关系（r=0.9996）;番石榴叶、苦瓜干和苦丁茶的平均回收率分别为99.92%（RSD=0.4%）、102.6%（RSD%=0.7%）和102.9%（RSD%=0.1%）。结论该方法准确、灵敏,可作为槲皮素HPLC分析的一个有效的方法。
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| 关 键 词： | 槲皮素 番石榴叶 苦瓜干 苦丁茶 高效液相色谱法 含量测定 |
Determination of quercetin in Guava leaf,Momordica charantiap,Kuding tea by high-performance liquid chromatography |
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| Deng Yadong,Yan Zhihong,Zhou Xudan. Determination of quercetin in Guava leaf,Momordica charantiap,Kuding tea by high-performance liquid chromatography[J]. , 2011, 0(3): 31-32 |
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| Authors: | Deng Yadong Yan Zhihong Zhou Xudan |
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| Affiliation: | .The Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510150 |
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| Abstract: | Objective To determine the content of quercetin with HPLC.Methods Content was measured by DiamonsiL C18（250mm×4.6mm,5μm）column,methanol-0.5% phosphoric acid（58：42）as mobile phase,detection wave length of 372nm flow rate 1ml/min.Results Quercetin in the range of 1.064～10.64μg/ml a good linear relationship（r=0.9996）,the average recovery of Guava leaf,Momordica charantiap,Kuding tea was 99.92%（RSD=0.4%）,102.6%（RSD%=0.7%）and 102.9%（RSD%=0.1%）,respectively.Conclusion The method is rapid,accurate and can be used for determination of quercetin by HPLC. |
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| Keywords: | Quercetin Guava leaf Momordica charantiap Kuding tea High performance liquid chromatography Content determination |
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