急性白血病患者骨髓组织血管新生与Bcl-2蛋白关系的研究

目的分析急性白血病患者骨髓病理切片中VEGF及Bcl-2蛋白的表达和微血管生成之间的相关性,并研究急性白血病患者骨髓组织血管新生与Bcl-2蛋白的关系。方法用免疫组织化学染色方法（EnVison）检测35例急性白血病患者和35例对照组骨髓病理切片中的VEGF,Bcl-2表达情况,并用第八因子相关抗原（FactorⅧ-related Antigen,FVIⅡ）标记血管内皮细胞,将免疫组化染色结果转化为平均光密度值进行定量分析。结果急性白血病患者Bcl-2蛋白阳性表达、MVD、VEGF含量均明显高于对照组,与对照组比较有显著性差异（P〈0.01）。通过对这3者进行两两相关性分析,结果MVD与VEGF表达呈正相关;Bcl-2蛋白阳性率与VEGF表达呈正相关;Bcl-2蛋白阳性率与MVD表达呈正相关。结论由于VEGF的表达,诱导产生更多的Bcl-2蛋白来降低急性白血病细胞凋亡,从而增加急性白血病的恶性程度,再加上本身促血管生成的作用,血管生成随之增加,从而增加了急性白血病的治疗难度。

The relationship between angiogenesis of myeloid tissue and Bcl-2 in acute leukemia

Objective To analysis the relationship among the expression of VEGF,Bcl-2 protein and MVD and demonstrate the relationship between angiogenesis and Bcl-2 in acute leukemia.Methods 35 pathological sections of bone marrow in acute leukemia were stained by immunohistochemical assay （EnVison） for Bcl-2 expression and VEGF expression,while microvessel density was calculated by means of marking Vascular endothelial cells with FⅧ（Factor Ⅷ-related Antigen）.The results of immunohistochemical staining was converted into the mean optical density through the computer analysis system.Results The expression of Bcl-2,MVD,VEGF in acute leukemia was significantly higher than control group（P0.01）.VEGF expression was correlated with MVD in acute leukemia.Bcl-2 expression was correlated with VEGF in acute leukemia.Bcl-2 expression was correlated with MVD in acute leukemia.Conclusion VEGF improves expression of Bcl-2 to lower acute leukemia Cell＇death,and increase malignant extent of acute leukemia.VEGF plays an important role in angiogenesis,then increased angiogenesis and remedial difficulty of acute leukemia.