金芪降糖片对糖尿病大鼠肾脏保护作用的实验研究

目的]观察金芪降糖片对糖尿病大鼠肾组织晚期糖化终末产物受体(RAGE)、转化生长因子-β1(TGF-β1)、血管内皮生长因子(VEGF)、结缔组织生长因子(CTGF)表达的影响。方法]测定造模后20周糖尿病组(DM组)、糖尿病给药组(DD组)和对照组(N组)大鼠24 h尿微量白蛋白定量(UMA)、平均血糖(APG)、甘油三酯(TG)、总胆固醇(TC)、内生肌酐清除率(Ccr)、RAGE、TGF-β1、VEGF、CTGF水平及肾脏形态学改变,评价金芪降糖片的肾保护作用。结果]与DM组相比,DD组平均血糖、TC差异无统计学意义,Ccr高于DM组,而UMA、TG、RAGE、TGF-β1、VEGF、CTGF水平降低,差异有统计学意义;光镜和电镜下,DD组肾组织损伤程度均轻于DM组。结论]金芪降糖片可以减轻糖尿病慢性肾损伤的程度,其肾保护作用可能与其降脂及降低RAGE、TGF-β1、VEGF、CTGF水平有关。

Experimental study of protective effect of Jinqi sugar-lowering tablet on kidney in rats with diabetes

Objective] To observe the effect of Jinqi sugar-lowering tablet on the expression of RAGE, TGF-β1, VEGF and CTGF in kidney of rats with diabetes. Methods] The level of RAGE,TGF-β1,VEGF and CTGF in kidney in group of rats with diabetes （group DM ）, group in diabetes with Jinqi tablet （group DD ） and control group （group N ） was determined at 20 weeks after inducing of diabetes. Their average plasma glucose （APG）, triglyceride （TG）, total cholesterol （TC）, endogenous creatinine clearance （Ccr） and urine microalbumin were recorded. Results] Compared to the group DM, the level of UMA,TG, RAGE, TGF-β1,VEGF and CTGF in group DD was lower （P〈0.05）, but the Ccr was higher. The level of APG and TC between group DD and group DM was not different. Renal pathological changes in group DD was better than that in DM group. Conclusion] Taking Jinqi Sugar-lowering tablet may decrease the renal damage of diabetes. The renal protection of Jinqi Sugar-lowering tablet is related with controlling blood sugar and it may be related with the inhibition of RAGE,TGF-β1,VEGF and CTGF.