UPLC法同时测定不同产地大黄中游离蒽醌的含量

[目的]建立超高效液相色谱法同时测定13批不同产地大黄中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素等5种游离蒽醌的含量。[方法]采用Waters BEH C18(2.1 mm×150 mm,1.8μm),以乙腈(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱[0~3 min,19%→25%A,3~10 min,25%→100%A],流速0.3 mL/min,柱温30℃,检测波长254 nm。[结果]大黄酸、大黄酚、大黄素、大黄素甲醚、芦荟大黄素分别在1.61~80.30,4.98~249.20,3.27~163.30,9.65~482.50,1.67~83.30μg/mL范围内呈现良好的线性关系,平均回收率分别为99.7%,100.4%,100.1%,98.9%,100.9%。相对标准偏差(RSD)分别为1.5%,0.6%,0.8%,0.4%,1.7%。[结论]该方法简便可行,重复性良好,可用于大黄药材的质量控制。

Simultaneous determination of free anthraquinones in Rhubarb fromdifferent habitats by UPLC

[Objective] To develop a UPLC method for simultaneous determination of five anthraquinones （Rhein, Chrysophanol, Emodin, Physcion and Aloe-emodin） in Radix et Rhizom a Rhei from different habitats. [Methods] The analysis was performed on Waters BEH C18 column （2.1 mm×150 mm, 1.8 μm） with a gradient mobile phase of acetonitrile （A）-0.1% Formic acid solution （B）（0N3 min, 19%→25% A, 3-10 min, 25%→100%A） at flow rate of 0.3 mL/min. The column was maintain at 30℃ and the detection wavelength was set at 254 nm. [Results] The linear ranges were 1.61 -80.30 μg/mL for Rhein, 4.98 N249.20 μg/mL for Chrysophanol, 3.27-163.30 μg/mL for Emodin, 9.65 -482.50 μg/mL for Physcion and 1.67-83.30 μg/mL for Aloe-emodin. The average recoveries of the five compounds were 99.7%, 100.4%, 100.1%, 98.9%, 100.9% and RSD were 1.5%, 0.6%, 0.8%, 0.4%, 1.7% respectively. [Conclusion] The rapid and accurate UPLC method was established. This method can be used to assess the quality of Rhubarb.