| 高效液相色谱法测定病毒清颗粒中黄芩苷含量 |
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| 引用本文： | 兰勇. 高效液相色谱法测定病毒清颗粒中黄芩苷含量[J]. 中国药业, 2010, 19(7): 23-24 |
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| 作者姓名： | 兰勇 |
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| 作者单位： | 湖南省怀化市第五人民医院,湖南,怀化,418000 |
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| 摘 要： | 目的建立测定病毒清颗粒中黄芩苷含量的高效液相色谱（HPLC）法。方法色谱柱为DiamonsilC18（250mm×4．6mm，5μm），以甲醇-水-冰醋酸（50：50：1）为流动相，检测波长为274nm。结果黄芩苷进样量在0．1034—1．0340μg范围内与峰面积线性关系良好，r=0．9992（n=5），平均加样回收率为99．30％，RSD为1．79％（n=6）。结论所用方法简便易行、准确、重现性好，可用于病毒清颗粒的质量控制。
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| 关 键 词： | 高效液相色谱法 病毒清颗粒 黄芩苷 含量测定 |
Determination of Baicalin in Bingduqing Granules by HPLC |
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| Lan Yong. Determination of Baicalin in Bingduqing Granules by HPLC[J]. China Pharmaceuticals, 2010, 19(7): 23-24 |
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| Authors: | Lan Yong |
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| Affiliation: | Lan Yong (Fifth People＇s Hospital of Huaihua City, Huaihua, Hunan, China 418000) |
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| Abstract: | Objective To establish a HPLC determination method of baicalin in Bingduqing Granules. Methods The Diamonsil C18 column was used with methanol-water-glacial acetic acid （50：50：1） as the mobile phase, the detection wavelength was 274 nm. Results The good linear was obtained in the range of 0. 103 4- 1. 034 0 μg（ r =0. 999 2）. The average recovery rate was 99.30%, RSD = 1.79% （n =6）. Conclusion The method is simple, accurate and reproducible, which can be used for the quality control of Bingduqing Granules. |
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| Keywords: | HPLC Bingduqing granules baicalin content determination |
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