NOV对人神经干细胞增殖和分化的影响

目的 探讨NOV蛋白对人神经干细胞(HNSCs)增殖和分化的影响。方法 NOV基因重组质粒转染COS—7细胞，收集COS-7细胞和NOV基因修饰COS—7细胞的条件培养液(COS—CM和NOV—CM)，作用于培养的HNSCs，^3H—TdR掺入液闪检测HNSCs的增殖，免疫细胞化学和流式细胞仪检测HNSCs的分化。结果 (1)NOV—CM能明显促进HNSCs对^3H—TdR的摄入，说明NOV—CM能明显促进HNSCs的增殖，另外NOV—CM的促细胞增殖作用具有一定的量效关系；(2)免疫细胞化学和流式细胞仪结果显示，NOV—CM促进HNSCs向神经元方向分化。结论 NOV蛋白可能具有促进HNSCs增殖和分化的作用。

Effects of NOV protein on proliferation and differentiation of human neural stem cells

Objective To explore effects of nephroblastoma overexpression gene(NOV) protein on proliferation and differentiation of human neural stem cells(HNSCs). Methods After NOV was transfected into COS-7 cells with liposome method, conditioned mediums(CMs) of COS-7 cells and COS-7 cells modified by NOV gene were collected (COS-CM and NOV-CM, respectively). Proliferation of HNSCs cultured in the CMs was detected with incorporation of 3 H-TdR, and differentiation of HNSCs was examined by using immunocytochemistry and flow cytometry. Results (1)NOV-CM markedly promoted incorporation of 3 H-TdR to cultured HNSCs in a concentration-dependent manner, indicating that NOV-CM promotes proliferation of HNSCs. (2) NOV-CM increased differentiation of HNSCs to neurons, but decreased it to astrocytes. Conclusion NOV protein could promote proliferation and differentiation of HNSCs.