SPP对人脐静脉内皮EA.hy926细胞表达粘附分子的作用

目的:通过研究在N,N-二甲基鞘氨醇(DMS)作用下,人脐静脉内皮EA.hy926细胞株(EA株)表达粘附分子CD54(ICAM-1)和CD62p(PS)的变化,探讨第二信使1-磷酸鞘氨醇(SPP)参与动脉粥样硬化(AS)粘附过程的机制。方法:采用流式细胞仪分群作用衡量EA细胞株和单核细胞之间的粘附率,用细胞免疫组化法检测DMS作用于EA株后引起的粘附分子的表达。结果:随着DMS作用剂量或作用时间的增加,CD54与CD62p的表达均减少,同时粘附率也相应地下降。结论:AS早期的粘附过程可能是通过SPP来转导信息,以促进粘附分子表达。

Effect of N, N-dimethylsphingosine on the expression of adhesion molecules in human umbilical vein endothelial cell line EA.hy926

AIM: To investigate whether and how N, N-dimethylsphingosine (DMS) plays a role in modulating the adhesion of monocytes to vascular endothelial cells, and identify whether human umbilical vein endothelial cell line EA.hy926 take place of the vascular endothelial cells.METHODS: Adhesion ratio was measured by flow cytometry, and immunohistochemistry was used to detect the expression of ICAM-1 and P-selectin in HUVEC: EA.hy926 cells after the effect of DMS. RESULTS: DMS inhibited the adhesion of monocytes to HUVEC: EA.hy926 cells in a time-dependent and concentration-dependent manner by reducing the expression of ICAM-1 and P-selectin. CONCLUSIONS: DMS reduced adhesion molecule expression in vascular endothelial cells. DMS may be an important contributor to reduce adhesion ratio, suggesting that DMS plays a negative role in proinflammatory and immune functions of the modified vascular endothelial cells during atherosclerosis and restenosis.