| 牛血清白蛋白的抗血清在免疫学沉淀反应实验中的应用 |
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| 引用本文： | 蔡玲斐. 牛血清白蛋白的抗血清在免疫学沉淀反应实验中的应用[J]. 医学理论与实践, 2005, 18(3): 254-255 |
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| 作者姓名： | 蔡玲斐 |
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| 作者单位： | 杭州师范学院医学院形态中心实验室,浙江省杭州市,310012 |
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| 摘 要： | 目的 :制备牛血清白蛋白 (BSA)抗血清 ,以探讨获取高效价抗血清的方法。方法 :用BSA配制一定的浓度 ,免疫家兔 ,以制备兔抗 -BSA血清。利用免疫电泳、双向琼脂扩散试验分析和测定抗血清的效价。结果 :双向琼脂扩散在BSA 0 . 5g/mL的浓度时 ,测得抗血清效价为 1∶32 ,对流免疫电泳结果显示BSA 0. 5g/mL与抗BSA在 1∶16时形成一条清晰可见的沉淀线。结论 :BSA免疫家兔可获取效价较高的抗血清。
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| 关 键 词： | 牛血清白蛋白(BSA) 效价 沉淀线 免疫 |
| 文章编号： | 1001-7585(2005)03-0254-02 |
| 修稿时间： | 2004-09-09 |
Application of Antiserum of Bovine Serum Albumin in Immunoprecipitation Reaction Experiment |
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| CAI Lingfei. Application of Antiserum of Bovine Serum Albumin in Immunoprecipitation Reaction Experiment[J]. The Journal of Medical Theory and Practice, 2005, 18(3): 254-255 |
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| Authors: | CAI Lingfei |
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| Abstract: | Objective: To prepare antiserum of bovine serum albumin (BSA) for obtaining high titer antiserum. Methods: The serum of anti-BSA was prepared by immunizing rabbits with BSA. The titer of antiserum was detected by immunoelectrophoresis and double immunodiffusion. Results: The titer of antiserum was 1:32 in double immunodiffusion and counter immunoelectrophoresis showed a clear precipitation line in the ratio 1:16 for BSA:anti-BSA (all at 0.5g/ml BSA).Conclusion: The higher titer of antiserum is obtained by immunizing rabbits with BSA. |
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| Keywords: | Bovine serum albumin(BSA) Titer Precipitation line Immunity |
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