转PTEN 基因对人结肠癌细胞的抑制作用

 目的 探讨PTEN基因转染对结肠癌LoVo细胞的抑制作用. 方法 将人野生型及突变型PTEN cDNA通过脂质体导入结肠癌细胞LoVo中,并用RT-PCR、Western blot、流式细胞术检测细胞的PTEN mRNA和蛋白质表达.用流式细胞仪、DNA梯形带和MTT法分析5-Fu对转染前后LoVo细胞抑制周期和诱导凋亡的能力. 结果 转染野生型PTEN基因可明显增强结肠癌LoVo细胞的PTEN表达,5-Fu对转染后的LoVo细胞抑制细胞周期和诱导凋亡的作用明显增强.突变型在结肠癌细胞LoVo中有表达但无明显抑制作用. 结论 转染的PTEN基因可显著上调结肠癌LoVo细胞的PTEN表达,促进细胞凋亡,联合应用PTEN基因转染和5-Fu可获得协同的细胞毒作用.

Inhibition of Transfected PTEN on Human Colon Cancer

Objective To study the inhibitory effect of transfected PTEN on LoVo cells. Methods The human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analysized by Western blot and flow cytometry. Before or after transfection, the activities of 5-Fu in inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT. Results PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cell. Conclusion Transfected PTEN remarkably up-regulated PTEN expression in LoVo cells and promoted the apoptosis. PTEN transfection associated with 5-Fu treatment had cooperatively cytotoxic effect.