细胞增殖指标对判断脑膜瘤生物学行为的意义

运用ＤＮＡ图像分析（ＩＡＴ），核仁组成区嗜银染色技术（ＡｇＮＯＲ），增殖细胞核抗原（ＰＣＮＡ）及Ｋｉ－Ｓ单克隆抗体免疫组化染色对９３例脑膜瘤外科切除标本进行观察。结果显示恶性腋膜瘤（ＭＭ）、有复发的良性脑膜瘤（ＲＢＭ）、有局部浸润的良性脑膜瘤（ＩＢＭ）增殖能力高于无复发、无浸润的单纯良性脑膜瘤（ＳＢＭ）（Ｐ＜０．０１），良性脑膜瘤的复发或浸润行为与细胞增殖潜能有关。上述四种方法中Ｋｉ－Ｓ是一种敏感性较高的简便方法：ＡｇＮＯＲ不仅敏感性高，且重复性和稳定性好。结果进一步表明，当ＡｇＮＯＲ颗粒数大于或等于２．８时，良性脑膜瘤复发的可能性较大（Ｐ＜０．０５）。

Cell prolification indices and their values in the biological behaviours of meningioms

A study was made on 93 surgical specimens of meningiomas.The specimens included 21 malignant meningiomas(MM), 25 benign meningiomas with recurrence after completely surgical removal (RBM). 12 benign meningiomas with invasion into dura , skull or muscle (IBM)and 35 simple benign meningiomas without recurrence after surgical treatment 6 or more years(SBM). Four methods were used, namely , DNA image analysis technology(IAT) , proliferating cell auclear antigen(PCNA) , Ki - S1 monoclonal antibodies immunohistochemical staining and argyrophilic nucleolar organizer regions (AgNOR) in order to study the characteristics of meningioma is cell kinetics and their values in biological behaviours.The results indicate:(1)The proliferating potential of the MM. RBM and IBM is much greater than that of the SBM(P<0.01) and the benign meningioma's recurrence or invasion is related to its proliferating ability;(2) The DNA, PCNA, Ki - S1 and AgNOR are all useful to assess the meningioma's proliferating potential , and are significantly related to each other(P<0.01).Among the methods, Ki - S1 is the simplest and most sensitive . AgNOR is not only sensitive but also repeatable;(3) when benign meningima's AgNOR number per cell nucleus is 2.8 or more the meningioma is more likely to recur (P< 0. 05).