| 一种简便快速筛选双位点单克隆抗体方法的建立 |
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| 引用本文： | 王登顺,黄卫,白雪峰. 一种简便快速筛选双位点单克隆抗体方法的建立[J]. 细胞与分子免疫学杂志, 1993, 0(2) |
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| 作者姓名： | 王登顺 黄卫 白雪峰 |
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| 作者单位： | 北京514医院实验医学中心肿瘤研究室，北京514医院实验医学中心肿瘤研究室，第三军医大学在本室进修的研究生 北京 100101，北京 100101 |
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| 摘 要： | 本文建立了一种无需对单克隆抗体(McAb)进行纯化和酶标记,利用市售酶标记抗体既可筛选双位点McAb的简易方法。其基本原理是,首先将待筛选McAb与酶标抗体制成McAb-酶标抗体复合物,再以此复合物作示踪剂进行夹心ELISA。若夹心孔OD492值高于第一、二阴性对照孔之和者, 提示所筛选McAb可能为双位点抗体。文中还对应用该方法时的注意事项进行了讨论。
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| 关 键 词： | 抗体 单克隆 夹心酶联免疫吸附试验 双位点抗体 |
Establishment of Rapid and Convenient Sandwich ELISA for Screening Two-site Monoclonal Antibody |
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| Wang Dengshun,et al.. Establishment of Rapid and Convenient Sandwich ELISA for Screening Two-site Monoclonal Antibody[J]. Chinese journal of cellular and molecular immunology, 1993, 0(2) |
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| Authors: | Wang Dengshun et al. |
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| Abstract: | In the present study, a convenient procedure for screening two-site McAb was established using commercially available enzyme-labelled antibodies. The procedure did not require that McAb be purified and labelled with enzyme. A complex of McAb and enzyme-labelled antibody was first prepared as a tracer, and then determination was performed by sandwich ELISA. If optical density (0. D.) value at 492 nm in sandwich well was higher than the sum of the two O. D. values at 492 nm in No. 1 and No.2 negative control wells, the McAb screened may be a two site antibody. Moreover, points for applying the procedure have been discussed. |
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| Keywords: | antibody monoclonal sandwich ELISA two site monoclonal antibody |
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