小儿肺炎支原体感染的血清学检测及临床应用

目的:应用敏感、特异性强的检测方法检测呼吸道感染患儿血清肺炎支原体(MP)特异性抗体IgM(抗MP—IgM),协助临床早期诊断MP感染及早期治疗,缩短病程。方法:日本富士肺炎支原体检测试剂—SERODIA—MYCOⅡ凝集试验。结果:1772例呼吸道感染患儿检出MP—IgM阳性389例,检出率为21.9%。年龄<3岁、3～6岁、6～13岁3个年龄组患儿的MP—IgM阳性检出率分别为14.5%(147/1012),30.5%(139/456)和33.9%(103/304)。取860例呼吸道感染患儿,符合临床支原体诊断标准有的205例,抗MP—IgM阳性156例,阳性率为76.1%;不符合诊断标准的655例中,抗MP—IgM阳性仅为26例,假阳性率为4.0%。两组经统计χ2=48.2,P<0.01,有显著性差异。结论押SERODIA—MYCOⅡ凝集试验检测小儿MP感染具有操作简便、省时快捷、敏感度高、特异性强的优点,可作为早期诊断MP的客观指标。

Blood serum measurement and clinical observation of mycoplasma pneumonia infection in children

Objective To investigate the measurement of IgM of mycoplasma pneumonia (anti MP-IgM ) in children respiratory infection. Method The children charts of 1772patients with respiratory infection were reviewed. Japan Fuji mycoplasma pneumonia measurement reagent-SERODIA MYCOⅡ agglutination were used to measure serum MP-IgM. Results 21.9% patients (389cases)'s serum MP-IgM was positive. <3,3～6 and 6～13 years old children's serum MP-IgM positive level were 14.5%(147/1012),30.5%(139/456)and33.9%(103/304).In 806 cases of respiratory infection,205cases have been clinically diagnosed. MP-IgM positive rate was 76.1%(156cases);In other cases serum MP-IgM pseudoreation was 4.0%(26cases).The result showed significant difference (P<0.01). Conclusion SERODIA MYCOⅡ agglutination is the impersonal mark in early diagnosis of mycoplasma pneumonia infection in children.