血浆纤溶酶原与BDNF在抑郁模型大鼠海马区的表达及相关性研究

目的：探讨血浆纤溶酶原和脑源性神经营养因子（BDNF）与抑郁症的相关性，加深对抑郁症发病分子机制的认识，为抑郁症的诊断和治疗提供新的思路。方法建立慢性轻度不可预见性应激大鼠抑郁模型，检测大鼠快感缺乏，行为活动减少，体质量减轻等抑郁样症状。采用蛋白免疫印迹法分析大鼠海马组织血浆纤溶酶原和BDNF蛋白表达情况。结果血浆纤溶酶原和BDNF在对照组和观察组大鼠海马区表达的差异均有统计学意义（P＜0.01）。血浆纤溶酶原与BDNF的变化呈明显正相关（r＝0.65，P＜0.01），线性回归分析表明二者之间存在明显的依存关系（r2＝0.423），回归方程为：YBDNF ＝0.750X血浆纤溶酶原+0.201。结论应激可影响神经细胞的生长和存活，导致大鼠抑郁样行为的发生。同时检测到血浆纤溶酶原和BD N F的表达下降，BDNF与血浆纤溶酶原的表达呈正相关，提示血浆纤溶酶原和BDNF共同参与抑郁症的发病机制。

Study on plasminogen and BDNF expression in the hippocampus of depression rat mode and its relationship

Objective To study on the relationship between plasminogen and brain-derived neurotrophic factor(BDNF),and explain the molecular mechanism of depression ,then provide new clew for diagnosis and treatment of depression .Methods The chronic unpredictable mild depression rat mode was established ,then depression symptoms including absence of delight ,the decline of actions and activities ,and weight reduction of rat were tested .The levels of individual plasminogen and BDNF in hippocampus were determined by Western blot .Results The expression of BDNF and plasminogen in depression rat mode and control group was significantly different(P<0 .01) ,and there was a positive correlation between BDNF and plasminogen(r=0 .65 ,P<0 .01) .Accord-ing to the linear-regression analysis ,there was a dependence relationship between them(r2 =0 .423) .The equation of regression was YBDNF=0 .750XPlasminogen +0 .201 .Conclusion Stress could affect the growth and survival of nerve cell ,which lead to the depression behavior of rats ,meanwhile ,the decline of plasminogen and BDNF levels ,the positive correlation between them illustrate that plas-minogen and BDNF take part in the mechanism of depression .