| 肠杆菌基因间重复一致序列-聚合酶链反应技术在大肠埃希菌基因分型中的应用研究 |
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| 引用本文： | 俞雅萍,彭颖,吕建新.肠杆菌基因间重复一致序列-聚合酶链反应技术在大肠埃希菌基因分型中的应用研究[J].中国现代医学杂志,2006,16(14):2099-2101. |
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| 作者姓名： | 俞雅萍 彭颖 吕建新 |
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| 作者单位： | 温州医学院检验医学院,细胞与分子医学研究所,浙江,温州,325035 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的 应用ERIC-PCR技术对致尿路感染大肠埃希菌进行基因分型。方法 收集大肠埃希菌致尿路感染患者标本24例，以ERIC序列为引物结合位点，PCR扩增致病大肠埃希菌基因组。结果 24例菌株中23例得到阳性扩增，ERIC-PCR绘制的基因组指纹图条带清晰可辨且有一定的复杂度。结论 ERIC-PC技术可用于大肠埃希菌的基因分型，能够有效地鉴别不同的菌株。
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| 关 键 词： | 基因分型 大肠埃希菌 |
| 文章编号： | 1005-8982（2006）14-2099-03 |
| 收稿时间： | 2006-05-20 |
| 修稿时间： | 2006-05-20 |
Technique of ERIC-PCR applied in uropathogenic Escherichia coli genotyping |
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| YU Ya-ping,PENG Ying,LU Jian-xin.Technique of ERIC-PCR applied in uropathogenic Escherichia coli genotyping[J].China Journal of Modern Medicine,2006,16(14):2099-2101. |
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| Authors: | YU Ya-ping PENG Ying LU Jian-xin |
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| Institution: | Institute of Cellular and Molecular Medicine, School of Laboratory Medicine, Wenzhou Medical College, Wenzhou, Zhejiang 325035, P.R.China |
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| Abstract: | Objective] The new technique of ERIC-PCR was used to determine the genotype of uropathogenic Eseherichia coil Methods] ERIC sequences were synthesized and tested as opposing PCR primers in the amplication of uropathogenie Eseherichia coli genomic DNA, followed by 1% agarose gel electrophoresis. A total of 24 strains of pathogens were identified. Results] The bands patterns of 24 strains provided unambiguous DNA tigerprints of different E.coli species and strains. Conclusions] ERIC-PCR is an effective way to determine the genotype of E.coli and identify different strains. |
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| Keywords: | ERIC-PCR |
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