| 醛糖还原酶对211首糖尿病中药复方作用的筛选研究 |
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| 引用本文： | 柯向梅,张彦廷,张彦玲,吴中秋,张再康,牛彦平. 醛糖还原酶对211首糖尿病中药复方作用的筛选研究[J]. 上海中医药杂志, 2009, 43(3): 76-78 |
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| 作者姓名： | 柯向梅 张彦廷 张彦玲 吴中秋 张再康 牛彦平 |
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| 作者单位： | 河北医科大学中医学院中医系中医临床基础教研室,河北,石家庄,050091;华北制药集团新药研究开发公司天然药物研究室,河北,石家庄,050015;河北省赞皇县医院医务科,河北,石家庄,051230;河北医科大学第二医院,河北,石家庄,050000 |
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| 摘 要： | 目的通过醛糖还原酶高通量模型对211首中药复方的筛选,寻找治疗糖尿病的科研配方。方法从猪晶状体中提取醛糖还原酶,通过测定340nm处光密度的下降速率,间接测定醛糖还原酶的活性的方法建立的高通量醛糖还原酶筛模型的筛选,比较211首方剂的6 630个制备样品对醛糖还原酶的抑制作用。结果其中4首方中5组制备样品对醛糖还原酶有特异性的抑制作用。结论中药复方应用醛糖还原酶高通量模型筛选评价,简便快捷,可以优选得到科研配方。
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| 关 键 词： | 糖尿病并发症 醛糖还原酶 高通量筛选 中药复方 |
Screening of Actions of 211 Herbal Formulae for Diabetes with Aldose Reductase |
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| KE Xiang-mei ZHANG Yan-ting ZHANG Yan-ling WU Zhong-qiu ZHANG Zai-kang NIU Yan-ping.College of Traditional Chinese Medicine,Hehei Medical University.New Drug R,D Company,North China Pharmaceutical Group.Hospital of Zanhuang County,Hebei. Screening of Actions of 211 Herbal Formulae for Diabetes with Aldose Reductase[J]. Shanghai Journal of Traditional Chinese Medicine, 2009, 43(3): 76-78 |
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| Authors: | KE Xiang-mei ZHANG Yan-ting ZHANG Yan-ling WU Zhong-qiu ZHANG Zai-kang NIU Yan-ping.College of Traditional Chinese Medicine Hehei Medical University.New Drug R&D Company North China Pharmaceutical Group.Hospital of Zanhuang County Hebei |
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| Affiliation: | KE Xiang-mei1 ZHANG Yan-ting2 ZHANG Yan-ling3 WU Zhong-qiu1 ZHANG Zai-kang1 NIU Yan-ping41.College of Traditional Chinese Medicine,Hehei Medical University2.New Drug R&D Company,North China Pharmaceutical Group3.Hospital of Zanhuang County,Hebei4.The Second Hospital of Hebei Medical University |
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| Abstract: | Objective To select scientific herbal formulae for diabetes in high flux model by screening the actions of 211 herbal formulae with aldose reductase.Methods Extract aldose reductase from pig lens,determine indirectly the method of enzymatic active aldose reductase by measuring slowdown rates at optical density 340 nm and compare the repression of aldose reductase of 6630 prepared samples in 211 herbal formulae.Results Five groups of the prepared samples out of the 4 herbal formulae could specifically inhibi... |
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| Keywords: | Diabetic complication aldose reductase high flux screening herbal formula |
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