BALB/c裸鼠前列腺癌原位种植模型的超声应用研究

目的 应用高频彩色多普勒超声观察裸鼠前列腺癌原位种植模型肿瘤生长规律,为实验研究提供可靠的观察方法.方法 制作BALB/c裸鼠前列腺癌原位种植模型35只,2～8周每周随机抽取5只裸鼠行超声检查,记录肿瘤大小及声像图特征,每次超声检查结束后立即解剖裸鼠,分离前列腺组织,以HE染色光镜观察原位种植是否成功;根据超声测量肿瘤体积大小描绘肿瘤的生长曲线.结果 超声观察到的裸鼠前列腺癌种植肿瘤全部经HE染色证实.原位种植肿瘤超声表现为低回声,内部见彩色血流信号,7周后肿瘤内部出现无回声液化坏死区,并与周围组织边界不清.肿瘤生长曲线在4～6周上升最快,是肿瘤生长最旺盛期.结论 高频彩色多普勒超声是观察前列腺癌原位种植动物模型肿瘤生长规律的可靠方法.

Ultrasonic study on the orthotopic prostate tumors of BALB/c nude mice

Objective To evaluate the value of high frequency color Doppler ultrasound in detecting the growth of orthotopic prostate tumor in nude mice. Methods Thirty-five orthotopic prostate tumor models of BALB/c nude mice were established. High frequency color Doppler ultrasound was used to monitor the growth of tumors in five randomly selected BALB/c nude mice after 2 - 8 weeks. After examination,the nude mice were immediately sacrificed under narcotism. The tumors were dissected, fixed and embedded. The sections were stained by HE and reviewed under microscope as the gold standard of detecting the growth of orthotopic prostate tumors. The growth curves were recorded and analyzed. Results High frequency color Doppler ultrasound can be used to observe the size, resonance, blood supply and necrosis of tumors. The tumors grew fastest in 4～6 weeks after injection. After seven weeks, necrosis can be found in the center of the tumors while the boundary of the tumor was not clear. Conclusions High frequency color Doppler ultrasound can be a reliable method to monitor the growth of orthotopic prostate tumor in BALB/c nude mice.