抗淋球菌外膜蛋白单克隆抗体的研制及应用研究

目的：制备抗淋球菌外膜蛋白单克隆抗体，建立淋病快速诊断方法。方法：采用乙酸锂提取淋球菌ＷⅠ群和ＷⅡ／ＷⅢ群外膜蛋白，以此为抗原免疫ＢＡＬＢ／Ｃ小鼠，融合、筛选抗淋球菌外膜蛋白杂交瘤细胞。结果：筛选出抗ＷⅠ群外膜蛋白杂交瘤２株，分泌的单克隆抗体类别为ＩｇＧ１；抗ＷⅡ／ＷⅢ外膜蛋白杂交瘤２株，分泌的单克隆抗体类别为ＩｇＧ２ａ和ＩｇＧ２ｂ。腹水单克隆抗体效价达１０７以上。４株单克隆抗体与脑膜炎球菌等细菌无交叉反应。对５６例临床标本采用夹心ＥＬＩＳＡ法进行检测，结果发现敏感性达９７％，特异性达１００％。结论：该单克隆抗体可以用于淋病的快速诊断。

The Production and Application of Monoclonal Antibodies Against Outermembrane Protein of Neisseria Gonorrhoeae

Objective: Produce monoclonal antibodies against outer membrane protein of Neisseria gonorrhoeae and set up methods of rapidity diagnosis. Methods: The outer membrane protein of Neisseria gonorrhoeae WⅠ and WⅡ/WⅢ were extracted with lithium acetate buffer. Results: We got 4 strains hybrid cell, which of 2 hybrid cells producing monoclonal antibodies against outer membrane protein of WⅠ secreted IgG 1, and the other 2 hybrid cells producing monoclonal antibodies against outer membrane protein of WⅡ/WⅢ secreted IgG 2a and IgG 2b . The titers of antibodies in ascites were more than 10 7 non-cross reactions were found with the strains of N. meningitides etc. The ELISA test was evaluated against the culture method of N. gonorrhoeae. The clinical specimens were collected from 56 swabs. The ELISA had a sensitivity of 97% and specificity of 100% were compared with the culture method. Conclution: The monoclonal antibodies could be used for rapidity diagnosis of gonorrhea.