CO2气腹对肝硬变大鼠门脉血流影响的实验研究

目的　探讨CO2 气腹对肝硬变大鼠门脉血流量的影响。　方法　制作肝硬变大鼠模型 ,施加不同压力的气腹 ,分别测平均动脉压、门静脉压力、下腔静脉压、门静脉血流 ,计算门静脉阻力。　结果　平均动脉压、下腔静脉压在不同气腹压力下无明显变化 ,两组大鼠门静脉压力在 10mmHg时出现显著差异 ,随着压力的升高而升高。正常大鼠的门静脉血流量在 2 0mmHg下与 0mmHg下比较有显著差异 ,而肝硬变大鼠的门静脉血流量在 10mmHg下与 0mmHg下比较已有显著差异。正常大鼠门静脉阻力随着压力的升高在 10mmHg时出现显著差异 ,以后继续升高 ,肝硬化大鼠门静脉阻力随着压力的升高持续升高。　结论　CO2 气腹致肝硬化大鼠门静脉血流减少 ,且减少程度重于正常大鼠

Experimental Study on Influence of CO2 Pneumoperitoneum on portal venous flow in rats with cirrhosis

Objective To elucidate the effects of CO 2 pneumoperitoneum on the flow of portal vein in the rats with cirrhosis. Methods We replicated cirrhosis rat model and established CO 2 pneumoperitoneum of different pressures. The mean arterial pressure(MAP), portal pressure(PP), inferior vena cava pressure(IVCP), portal venous flow(PVF) were determined differently. The portal vascular resistance (PVR) was calculated. Results (1)MAP and IVCP did not change significantly at different pressures. PP changed significant at 10mmHg and increased with the rise of pressure in both groups. (2) The blood flow of portal vein in the control group decreased significantly at 20mmHg of pressure compared with the flow at 0mmHg of pressure, but in the cirrhosis group it decreased remarkably at 10 mmHg compared with the flow at 0mmHg. (3) The PVR in the control group showed the a significant differencte. At the same time, the PVR in the cirrhosis group increased continuously with the increasing pressure. Conclusions CO 2 pneumoperitoneum led to the decrease of PVF in the cirrhosis rats and the level of decrease was more significant than that in the control group.