RP-HPLC测定桑寄生及其寄主植物黄皮树盐酸小檗碱的含量

目的:测定桑寄生及其寄主植物中盐酸小檗碱的含量,研究寄主植物对桑寄生药材质量的影响。方法:采用甲醇-盐酸(100∶1)溶液超声提取样品中的盐酸小檗碱,并采用RP-HPLC法进行含量测定,选用Agilent C18柱(250 mm×4.6 mm,5μm),流动相:乙腈:水:磷酸:三乙胺(72∶28∶0.01∶0.05),流速:1.0 mL.min-1,柱温:室温,检测波长:284 nm。结果:不同寄主植物黄皮树及其桑寄生中盐酸小檗碱含量范围分别为0.14~7.58 mg.g-1和0.11~1.35 mg.g-1,桑寄生盐酸小檗碱含量最高可达到其寄主植物黄皮树盐酸小檗碱含量的132.10%。结论:寄主植物是影响桑寄生药材质量的关键,寄主植物不同桑寄生药材化学成分含量也不同。

Determination of Berberine in Taxilli Herba and Its Host Plants -Clausena lansium by RP-HPLC

This study was aimed to determine contents of berberine in Taxilli Herba and its host plants Clausena lansium,and further to explore the effect of host plants on Taxilli Herba.Berberine was extracted with CH3OH-HCL(100:1) solution by ultrasound.The content of berberine was determined by RP-HPLC.Agilent C18 column(250 mm × 4.6 mm,5 μm) was used for the determination,with a flow rate of 1.0 mL·min-1 and column temperature of 25℃.The mobile phase were acetonitrile-warter-phosphoric acid-triethylamine(72:28:0.01:0.05).The detection wave length was 284 nm.The results showed that contents of berberine in Taxilli Herba and its host plants-Clausena lansium were 0.11 mg·g-1-1.35 mg·g-1 and 0.14 mg·g-1-7.58 mg·g-1,respectively.The highest content of berberine in Taxilli Herba is as high as 132.10% as that in Clausena lansium.It was concluded that host-plants exert a very important effect on the quality of Taxilli Herba.With different host plants,the content chemical composition in Taxilli Herba were different.