六味地黄丸动态提取工艺优选

目的： 探讨强制循环动态提取工艺对六味地黄丸复方提取效果的影响。 方法： 以干膏率、马钱苷提取量为评价指标，在不改变本品现有标准中提取次数的前提下，采用正交试验考察加水量和提取时间对六味地黄丸动态提取工艺的影响，并与传统提取方法进行比较。采用HPLC测定马钱苷含量，流动相四氢呋喃-甲醇-乙腈-0.05%磷酸溶液（1：4：8：87），检测波长236 nm。 结果： 加水量对干膏率和马钱苷提取量的影响具有显著性差异，提取时间则无显著性差异。六味地黄丸的最佳动态提取工艺为加3倍量水提取2次，每次1 h；马钱苷平均提取量分别为1.068 mg·g^-1，平均干膏率25.75%。 结论： 优选的提取工艺稳定可行，具有节能、省时、提取率高等特点，适用于六味地黄丸的工业化生产。

Optimization of Dynamic Extraction Technology of Liuwei Dihuang Pills

Objective: To investigate influence of forced circulation dynamic technology upon extraction effect of Liuwei Dihuang pills. Method: With extraction ratio of loganin and dry extract ratio as indicators,under premise of extraction times without changing from the existing standard of the product,orthogonal design was used to investigate effects of the amount of adding water and extraction time on dynamic extraction technology of Liuwei Dihuang pills,and compared with traditional extraction methods.HPLC was established to determine the content of loganin with mobile phase of tetrahydrofuran-methanol-acetonitrile-0.05% phosphoric acid solution(1:4:8:87) and detection wavelength of 236 nm. Result: The amount of adding water had significant difference influence on extraction amount of loganin and dry extract ratio,but extraction time had no significant difference.Optimum dynamic extraction technology was as following:extracted twice with three times the amount of water,one hour per time;Average extraction amount of loganin was 1.068 mg·g^-1,average rate of dry extract was 25.75%. Conclusion: This optimized technology was stable and feasible with characteristics of energy saving,time-saving,high extraction rate and so on,it was suitable for industrial production of Liuwei Dihuang pills.