Ethanol-induced conditioned place preference, but not aversion, is blocked by treatment with d-penicillamine, an inactivation agent for acetaldehyde |
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Authors: | Laura Font Carlos M G Aragon Marta Miquel |
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Institution: | (1) área de Psicobiología, Universitat Jaume I, Campus de Riu Sec, Apartat 8029AP, Castellò, 12071, Spain |
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Abstract: | Rationale There is evidence to suggest that acetaldehyde is involved in the control of ethanol-seeking behavior and reward. d-penicillamine, a thiol amino acid, is a highly selective agent for the inactivation of acetaldehyde. Previous studies from
our laboratory have demonstrated that d-penicillamine prevents both behavioral stimulation induced by ethanol and acetaldehyde-produced locomotor depression in mice.
Objectives The contribution of ethanol-derived acetaldehyde to the affective effects of ethanol (preference and aversion) was assessed
using an unbiased place conditioning design.
Methods Male mice received four pairings of a distinctive floor stimulus (CS+: GRID+ or HOLE+) with injections of saline and ethanol
(2 g/kg) given before (preference) or after (aversion) the 5-min exposure to the place conditioning apparatus. A different
floor stimulus (CS−: GRID− or HOLE−), associated with saline-saline injections on alternate days, was presented. For a different
group of animals, the pairings with the CS+ were associated with saline and ethanol injections, but on alternate days, they
received d-penicillamine (50 or 75 mg/kg) and ethanol injections paired with the CS−floor stimulus. A 60-min preference test was carried
out 24 h after the last conditioning trial. A similar procedure was followed to test the effect of d-penicillamine on morphine (16 mg/kg) and cocaine-induced (20 mg/kg) conditioned place preference (CPP).
Results CPP and conditioned place aversion (CPA) were observed for ethanol, but d-penicillamine only blocked CPP. d-penicillamine, by itself, did not produce either rewarding or aversive effects. CPP observed for morphine and cocaine was
unaffected by d-penicillamine pretreatment.
Conclusions The results of the present study suggest that the selective inactivation of acetaldehyde blocked the rewarding, but not aversive,
effects of ethanol and support the role of this ethanol metabolite in the affective properties of ethanol. |
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Keywords: | Place conditioning Reward Aversion Ethanol metabolism Acetaldehyde d-penicillamine" target="_blank">d-penicillamine Mice |
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