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丙型肝炎病毒核心抗原检测技术的临床应用
引用本文:陈振球.丙型肝炎病毒核心抗原检测技术的临床应用[J].实用医技杂志,2010,17(1):14-16.
作者姓名:陈振球
作者单位:湖南省石门县人民医院,415300
摘    要:目的探讨丙型肝炎病毒(HCV)核心抗原检测酶联免疫吸附试验(ELISA)]法的临床应用价值。方法采用聚合酶链反应(PCR)-荧光探针法对临床HCV感染者血浆进行HCV-RNA检测,并用ELISA对标本进行HCV抗体和HCV核心抗原的检测。结果87例HCV感染者HCV-RNA的检出率为75%(65/87);HCV抗体的检出率为95%(83/87);HCV核心抗原的检出率为67%(58/87)。另有2例标本HCV抗体阴性,而HCV核心抗原阳性,HCV-RNA也为阳性。结论HCV核心抗原的检测对HCV感染的早期诊断有重要价值。

关 键 词:肝炎抗体  丙型  肝炎病毒RNA

Clinical application of the detection technique of hepatitis C virus core antigen
CHEN Zhen-qiu.Clinical application of the detection technique of hepatitis C virus core antigen[J].Journal of Practical Medical Techniques,2010,17(1):14-16.
Authors:CHEN Zhen-qiu
Abstract:Objective To disscuss the clinical application value of the method used in detection hepatitis C virus core antigen(HCVcAg) by enzyme-linked immunosorbent assay(ELISA) . Methods The HCV-RNA in the plasma samples from the people infected HCV were detected by PCR-fluorescence probing assay. The HCVcAg and HCV antibody(HCVAb) were detected by ELISA. Results The positive rate of HCV-RNA,HCVAb and HCVcAg in 87 patients were 74.7%(about 65 in 87) ,95.4%(about 83 in 87) and 66.7%(about 58 in 87) ,respectively. In ...
Keywords:Hepatitis C antibody  Hepacivirus RNA  
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