解脲、人型支原体固体和液体培养基方法比较

目的：探讨泌尿生殖道支原体培养试验中假阳性、假阴性结果的原因及改进的对策，研究临床检测解脲（Uu）和人型支原体（Mh）培养的方法。方法：对208例泌尿生殖道拭子直接用固体培养分离Uu和Mh，同时接种液体培养基作为对照。观察并记录24 h、48 h、72h固体培养基长出支原体菌落的阳性例数和液体培养阳性例数，并用统计学方法分析两种方法的差异。结果：固体培养基直接分离培养结果与液体培养在不同时间段所得到的结果，阳性率无统计学差异，99%的菌落于24 h～48 h之间可辨认。结论：固体培养基直接分离Uu和Mh具有鉴别准确，选择性强和菌落特征明显的优点，与液体培养相比更适用于支原体的临床检测。

Analysis of Isolation and Culture of Ureaplasma urealyticum and Micoplasma hominis by Solid Medium and liquid Medium

Objective To explore the genitourinary tract mycoplasma culture reason of false positive and false negative result in test and improvement countermeasures, to study the clinical detection of ureaplasma type (Uu) and mycoplasma (Mh) training Method 208 cases of urinary tract swab with solid separation culture Uu and Mh directly, inoculation fluid medium as control at the same time. Observe and record 24 h, 48 h, 72 h solid medium grow colonies mycoplasma positive cases and liquid culture positive cases, and statistical methods to analyze the differences of two methods. Results Solid medium direct separation of Uu and Mh have identified accurately, selectivity and the obvious advantages of colony characteristics, compared with the liquid culture is more suitable for clinical test of mycoplasma.