Stimulus-dependence and time-course of histamine and leukotriene release from chopped and dispersed human lung tissue |
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Authors: | A. Sydbom M. Kumlin S. -E. Dahlén |
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Affiliation: | (1) Department of Pharmacology, Karolinska Institutet, S-104 01 Stockholm, Sweden;(2) Department of Physiological Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden;(3) Department of Physiology, Karolinska Institutet, S-104 01 Stockholm, Sweden |
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Abstract: | The calcium ionophore A23187 and anti-human IgE provoked a dose-dependent release of histamine and cysteinyl-leukotrienes (LTC4, LTD4 and LTE4) from dispersed human lung cells. Optimal release of histamine peaked at 3 min after challenge whereas the release of cysteinyl-leukotrienes peaked at 30 min. The molar ratio between released histamine and cysteinyl-leukotrienes was approximately 1001. The ionophore, but not anti-IgE caused additional formation of LTB4 in the dispersed cells or the chopped lung, indicating that this chemoattractant is formed from cells not primarily activated by IgE-dependent mechanisms. Indomethacin failed to alter release of histamine or leukotrienes from dispersed cells, whereas further inhibition of lipoxygenases by NDGA abolished leukotriene formation and reduced the release of histamine. |
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