| 扩增精液与阴道液混合斑中Y染色体特异DNA重复序列的检测 |
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| 引用本文： | 梁景青,王伶,常明立. 扩增精液与阴道液混合斑中Y染色体特异DNA重复序列的检测[J]. 山西医科大学学报, 1998, 0(1) |
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| 作者姓名： | 梁景青 王伶 常明立 |
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| 作者单位： | 山西医科大学法医系，山西医科大学外语教研室 |
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| 摘 要： | 用ＤＮＡ扩增技术，检测阴道液和精液混合斑的Ｙ染色体特异ＤＮＡ重复序列，同时对混合斑的ＤＮＡ提取方法进行了改进，获得了理想的Ｙ染色体ＤＮＡ片段的扩增产物。方法简便，灵敏，准确。为鉴定混合斑提供新的检测手段
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| 关 键 词： | 精液混合斑 聚合酶链反应 Y染色体特异DNA重复序列 |
Determination of human semen in mixed stain by the DNA amplification technique |
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| Liang Jingqing ,Wang Ling ,Chang Mingli. Determination of human semen in mixed stain by the DNA amplification technique[J]. Journal of Shanxi Medical University, 1998, 0(1) |
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| Authors: | Liang Jingqing Wang Ling Chang Mingli |
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| Affiliation: | Liang Jingqing 1,Wang Ling 1,Chang Mingli 2 |
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| Abstract: | The determination of Y target DNA seauenses (Y repeat)in mixed stains of human semen and vaginal secretion by the DNA amplification technique was reported and method of extracting DNA was improved.Satisfied amplification products were obtained in mixed stains with the primers Y 1 1 and Y 1 2 .The method in identification of human semen,especially mixed stains of human semen and vaginal is sensitive,exact and simple. |
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| Keywords: | semen mixed stain polymerase chain reaction Y specific repeat sequence |
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