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| 粪便脱落细胞多基因变异与大肠癌早期诊断的关系 | |
| 引用本文： | 盛希忠,申泮远,穆庆岭,王教华. 粪便脱落细胞多基因变异与大肠癌早期诊断的关系[J]. 山东医药, 2001, 41(14): 4-6 |
| 作者姓名： | 盛希忠 申泮远 穆庆岭 王教华 |
| 作者单位： | 1. 山东省千佛山医院 2. 山东省立医院 3. 淄博市矿务局双山医院 |
| 摘 要： | 采用PCR－SSCP分析技术，检测40例大肠癌癌组织中抑癌基因APC第11外显子、MCC等10外显子和癌基因K－ras第1显子的变异情况，并在20例大肠癌患者粪便中进行三种基因的检测。结果：APC第11外显子和MCC第10外显子在A／B期变异率明显高于C／D期，K－ras第1外显子突变率在粘液癌中明显高于腺癌，且随分化程度降低有降低的趋势；在粪便中均检测到与肿瘤组织中三种基因相应的变异。认为检测烘便脱落细胞基因变异可用于大肠癌早期诊断,APC/MC和K-ras可用于估计大肠癌预后。 |
| 关 键 词： | 结直肠肿瘤 基因突变 粪便 大肠癌 PCR－SSCP |
| 修稿时间： | 2001-05-28 |
The relationship between alterations of multiple genes in stools from patients with colorectal carcinoma and the role in early diagnosis |
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| Sheng Xizhong,Shen Panyuan,Mu Qingling. The relationship between alterations of multiple genes in stools from patients with colorectal carcinoma and the role in early diagnosis[J]. Shandong Medical Journal, 2001, 41(14): 4-6 | |
| Authors: | Sheng Xizhong Shen Panyuan Mu Qingling |
| Abstract: | The changes of exon 11 of APC (adenomatous polyposis coli) gene, exon 10 of MCC(mutated in colorectal carcinoma)gene and exon 1 of K-ras (Kirsten rat sarcoma)gene were separately assayed with PCRSSCP(polymerase chain reaction-single strand conformation polymorphism) technique in 40 samples of colorectal carcinoma and 20 samples from stools of patients with colorectal carcinoma. Results showed the alterative rate of exon 11 of APC and exon 10 of MCC were significantly higher in stage A/B than in stage C/D. The mutation rate of exon 1 of K-ras were significantly higher in mucinous carcinoma than in adenocarcinoma, and the rate had a decreasing tendency with the differentiation decreased. It is thought that detection of APC/MCC and K-ras genes might be used in early diagnosis and prognosis of colorectal carcinoma. |
| Keywords: | Colorectal neoplasm Gene Mutation Feces |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |