CD105和CD34染色在胃癌组织新生血管检测中的差异及其意义

①目的比较以CD105和CD34为血管内皮细胞标记物在胃癌新生血管研究中的差异，并分析其意义。②方法采用免疫组化SABC法分别对55例新鲜切除的胃癌组织标本进行CD34和CD105染色。并比较其肿瘤的微血管密度（IMD）。③结果CD105染色极易将胃癌组织切片中的新生血管与非新生血管区分开来；而CD34染色切片中非新生血管亦有着色。以CD34为内皮细胞标记物计得的肿瘤内IMD值显著高于CD105组（t=12．08，P〈0．001）；以CD34计数肿瘤远处转移组IMD值与无肿瘤转移组无明显差异（t=0．59，P〉0．05）；而以CD105计数所得肿瘤远处转移组IMD值高于无远处转移组（t=2．58，P〈0．05）。④结论CD105是一种较CD34更好的肿瘤性血管内皮细胞标记物，适用于肿瘤新生血管的研究。

DIFFERENCE AND SIGNIFICANCE OF CD105 AND CD34 IN DETECTING NEWBORN BLOOD VESSELS IN GASTRIC CARCINOMA

ObjectiveTo compare the two endothelium cell markers, CD105 and CD34, in detecting the newborn blood vessels of gastric carcinoma and to analyze its significance. MethodsCD34 and CD105 of 55 fresh cases of gastric carcinoma were detected by using immunohistochemistry SABC and intratumoral microvessel density (IMD) were counted.ResultsNewborn blood vessels and normal blood vessels could be clearly distinguished in CD105 stained slides, but not in CD34 stained slides. IMD in CD34 stained slides was significantly higher than those in CD105 stained slides (t=12.08,P<0.001). IMD of CD105 stained slides had clearly relationship with gastric carcinoma distance metastasis (t=2.58,P<0.05). ConclusionCD105 is more suitable than CD34 in studying the newborn blood vessels of malignant tumor.