| HPLC法测定双香排石颗粒中甘草酸的含量 |
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| 引用本文： | 于立军. HPLC法测定双香排石颗粒中甘草酸的含量[J]. 中国药房, 2007, 18(18): 1404-1405 |
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| 作者姓名： | 于立军 |
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| 作者单位： | 南京军区南京总医院疗养区药械科,南京市,211131 |
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| 摘 要： | 目的:建立以HPLC法测定双香排石颗粒中甘草酸含量的方法。方法:色谱柱为Lichrospher C18(250mm×4.6mm,5μm),流动相为甲醇-0.2mol·L-1醋酸铵-冰醋酸(67∶33∶1),检测波长为250nm,流速为0.8mL·min-1。结果:甘草酸铵盐进样量在0.4~6.0μg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为98.0%,RSD=1.85%(n=9)。结论:本方法简便、易行、重现性好,可用于双香排石颗粒的质量控制。
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| 关 键 词： | 高效液相色谱法 双香排石颗粒 甘草酸 含量测定 |
| 文章编号： | 1001-0408（2007）18-1404-02 |
| 收稿时间： | 2007-01-06 |
| 修稿时间： | 2007-01-062007-04-21 |
Determination of Glycyrrhizin in Shuangxiangpaishi Granules by HPLC |
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| YU Lijun. Determination of Glycyrrhizin in Shuangxiangpaishi Granules by HPLC[J]. China Pharmacy, 2007, 18(18): 1404-1405 |
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| Authors: | YU Lijun |
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| Abstract: | OBJECTIVE: To determine the content of glycyrrhizin in Shuangxiangpaishi granules by HPLC. METHODS: The Glycyrrhizin was separated on Lichrospher C18( 250mm× 4. 6mm, 5μ m) with mobile phase consisted of methanol-0. 02mol· L-1 ammonium acetate-glacial acetic acid ( 67∶ 33∶ 1) at a flow rate of 0. 8mL· min-1. The detective wavelength was set at 250nm. RESULTS: The linear range of Ammonium glycyrrhizinate was 0. 4～ 60μ g ( r=0. 999 9) , and its average recovery rate was 98. 0% , RSD=1. 85% ( n=9) . CONCLUSION: The method is simple, feasible and reproducible, and suitable for the quality control of Shuangxiang paishi granules. |
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| Keywords: | HPLC Shuangxiangpaishi granules Glycyrrhizin Determination of content |
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