基质细胞衍生因子-1对脐血单个核细胞黏附作用的影响

目的探讨基质细胞衍生因子-1（SDF-1）对脐血单个核细胞（MNC）黏附作用的影响，揭示SDF-1和干细胞归巢的相关性，从而为基础研究以及临床上造血干细胞移植提供依据。方法从脐血中分离出MNC，用纤连蛋白包被法和MTF法检测在不同浓度SDF-1下的黏附率；然后，体外培养MNC，分别在第0、4、7、10、14天收集细胞进行黏附率检测，同时通过流式细胞术检测VLA-4表达量。结果SDF-1质量浓度分别为50、100、150、200、250ng／ml时，新鲜脐血MNC黏附率分别为（110．2±2．10）％、（128．3±2．09）％、（141．6±2．62）％、（160．8±3．84）％、（162．3±3．60）％、（165．4±2．73）％、（165．1±2．33）％。体外培养MNC第0、4、7、10、14天，黏附分子VLA-4表达量分别为26．37±4．35、40．67±3．59、25．99±4．72、22．82±3．75、17．29±2．49，MNC黏附率分别为（160．2±6．38）％、（194．6±6．09）％、（147．5±6．86）％、（144．7±4．96）％、（118．1±3．64）％。细胞黏附率与VLA-4表达量之间相关系数为0．826。结论随着SDF-1浓度的增高，MNC黏附率递增，但当SDF-1浓度达到100ng／ml，细胞黏附率达稳定，继续增高SDF-1浓度，细胞黏附率变化不显著。体外培养初期VLA-4的表达和MNC黏附率均有所升高，随着培养时间的延长又都逐渐降低。MNC黏附率与VLA-4表达量间存在正相关。

Effect of stromal cell-derived factor-1 on the adherence function of mononuclear cells

Objective To investigate the effects of stromal cell-derived factor-1 (SDF-1) on the adherence of mononuclear cells (MNC). Methods Mononuclear cells were isolated from cord blood and the adherent rate was assayed by using MTT with fibronectin coated in different concentration of SDF-1. Then the expression of adherent molecule VLA-4 by FACS as well as the adherent rate when MNC were cultured in vitro. Results The adherent rate of fresh cord blood MNC was (110.2±2.10) %, (128.3±2.09) %, (141.6±2.62) %, (160.8±3.84) %, (162.3±3.60) %, (165.4±2.73) %, (165.1±2.33) %. When the mass concentration of SDF-1 was 50, 100, 150, 200, 250 ng/ml. When MNC were cultured in vitro,the expression of VLA-4 was 26.37te was (160.2±6.38) %, (194.6±6.09) %, (147.5±6.86) %, (144.7±4.96) %, (118.1±3.64) %. The correlation coefficient between the adherent rate and the expression of VLA-4 is 0.826. Conclusion The adherent rate of fresh cord blood MNC increases along with the concentration of SDF-1, however, it tends to be stable when the concentration of SDF-1 reaches 100 ng/ml. When MNC were cultured in vitro with hemopoietic growth factors, the expression of VLA-4 and the adherent rate of MNC increased in the initial stage, however, the expression of VLA-4 and the adherent rate of MNC both decreased gradually along with time extending. There is positive correlation between the adherent rate of MNC and the expression of adherent molecule VLA-4.