| 脂多糖对非酒精性脂肪性肝炎大鼠肝细胞凋亡的影响 |
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| 引用本文： | 郭建红,杨丽红. 脂多糖对非酒精性脂肪性肝炎大鼠肝细胞凋亡的影响[J]. 山西医科大学学报, 2011, 42(7): 529-531,611. DOI: 10.3969/J.ISSN.1007-6611.2011.07.001 |
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| 作者姓名： | 郭建红 杨丽红 |
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| 作者单位： | 山西医科大学病理学教研室,太原,030001 |
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| 基金项目： | 山西省自然科学基金资助项目 |
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| 摘 要： | 目的通过观察非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,NASH)发生发展过程中LPS对肝细胞凋亡的影响,初步探讨LPS与NASH发病机制的内在联系。方法 30只雄性Wistar大鼠随机分为LPS组、NASH组、LPS+NASH组。LPS组饲以普通饮食,其余两组饲以高糖高脂饮食。LPS组和LPS+NASH组经皮下隔日注射LPS 0.5 mg/kg。于实验第9周末处死动物,制备肝组织切片,观察肝组织病理改变、计数凋亡细胞;测定血浆内毒素水平和丙氨酸转氨酶(alanine amino trnsferase,ALT)活性、肿瘤坏死因子-α(TNF-α)水平。结果 LPS+NASH组动物肝组织切片凋亡细胞数、血浆ALT、TNF-α水平与NASH组比较均显著增高(P<0.05)。结论 LPS可明显加重高糖高脂所致的NASH。LPS可能通过上调TNF-α水平、诱导肝细胞凋亡,从而促进NASH的发展过程。
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| 关 键 词： | 脂多糖 非酒精性脂肪性肝炎 凋亡 肿瘤坏死因子-α |
Effect of LPS on hepatocyte apoptosis in rats with nonalcoholic steatohepatitis |
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| GUO Jian-hong,YANG Li-hong. Effect of LPS on hepatocyte apoptosis in rats with nonalcoholic steatohepatitis[J]. Journal of Shanxi Medical University, 2011, 42(7): 529-531,611. DOI: 10.3969/J.ISSN.1007-6611.2011.07.001 |
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| Authors: | GUO Jian-hong YANG Li-hong |
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| Affiliation: | GUO Jian-hong,YANG Li-hong (Dept of Pathology,Shanxi Medical University,Taiyuan 030001,China) |
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| Abstract: | Objective To investigate the effect of lipopolysaccharide(LPS) on hepatocyte apoptosis in the development of nonalcoholic steatohepatitis(NASH),and to explore the role of LPS in the pathogenesis of NASH. Methods Thirty male Wistar rats were assigned randomly into 3 groups:LPS group,NASH group and LPS+NASH group.The rats were given normal diet in LPS group,high-sucrose and high-fat diet in NASH group and LPS+NASH group.And the rats in LPS group and LPS+NASH group were additionally injected with LPS 0.5mg/kg ... |
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