CIK细胞治疗慢性乙型肝炎的疗效观察

目的 观察自体细胞因子诱导的杀伤(cytokine-induced killer,CIK)细胞治疗慢性乙型肝炎的疗效及安全性,探索慢性乙型肝炎治疗的新策略.方法 采集32例e抗原阳性的慢性乙型肝炎患者外周血单个核细胞,在体外经干扰素γ、白细胞介素-2、抗CD3单克隆抗体等细胞因子诱导成CIK细胞,分次自体回输,观察治疗...

Treatment with autologous cytokine-induced killer cells in patients with chronic hepatitis B

Objective To evaluate the safety and efficacy of the treatment with autologous cytokine-induced killer(CIK) cells in patients with chronic hepatitis B(CHB) and to investigate a new therapeutic strategy for CHB.Methods Peripheral blood mononuclear cells(PBMCs) were isolated from 32 HBeAg-positive CHB patients,and then induced with cytokines such as interferon(IFN) γ,interleukin(IL)-2 and monoclonal antibody(mAb) against CD3 to CIK cells in vitro.The autologous CIK cells obtained were infused back to the individual patient.Liver function parameters,serum markers and HBV DNA levels were observed and com-pared before treatment and at week 1,4 and 12 after treatment.Meanwhile,adverse reactions and kindney function were evaluated.Results CIK cells were induced successfully in 32 patients.During the treatment,no obvious adverse reactions were seen except fever in most of the patients when and after CIK cells were infused.There were no significant differences in kidney function and hemocytes.ALT and AST levels improved significantly after treatment.At week 1,4 and 12 after treatment,ALT normalization rates were 31.3%,62.5% and 75.0%,AST normalization rates 53.1%,65.6% and 81.3%,HBeAg negative rates 50.0%,59.4% and 62.5%,and HBeAg seroconversion rates 18.8%,28.1% and 31.3%,respectively.HBV DNA levels didn’t show any significant change at week 1 after treatment,and decreased significantly as time went by.As compared with baseline levels,12.5%,31.2% and 53.1% of the patients had a reduction of 2 log10 in HBV DNA levels and 3.1%,12.5% and 34.3% of the patients had HBV DNA levels be-low the limit of detection at week 1,4 and 12 after treatment.Conclusions Treatment with autologous CIK cells is safe and effective in CHB patients during 12 weeks after treatment,and especially as time goes by,HBV DNA levels decrease gradually,which sug-gests sustainable immune response.