厦门地区汉族人PTPN22C1858T基因多态性与类风湿关节炎的关系研究

目的 建立TaqMan-MGB探针实时荧光聚合酶链反应(PCR)方法,研究福建厦门地区汉族人类风湿关节炎(RA)与PTPN22基因C 1858T多态性的相关性.方法 应用病例对照法,收集100例RA患者和100名健康人血液标本,利用TaqMan-MGB探针实时荧光PCR方法检测PTPN22C1858T基因多态性.结果 ①成功建立TaqMan-MGB探针实时荧光PCR技术检测PTPN22C1858T基因多态性.②PTPN22基因1858位点在福建厦门地区汉族人中仅存在C等位基因,未检测到T等位基因.结论 厦门地区汉族人不存在PTPN22 1858T等位基因,PTPN22 C1858T多态性与RA可能不存在关联.

Abstract：

Objective To assess the correlation between FFPN22 C1858T polymorphism and rheumatoid arthritis(RA)in Han people in Xiamen area by TaqMan-MGB real-time PCR．Methods A casecontrol study was carried out in Xiamen Han population．Their blood samples(100 RAs and 100 controls respectively)were collected and the PTPN22 C1858T polymnrphism was tested by TaqMan-MGB real-time PCR．Results ①A technique of TaqMan-MGB teal-time PCR was established to investigate PTPN22 C1858T polymorphism；② Onlv 1858 C allele was presented in all the RAs and controls，and no T allele was detected．Conclusion There is no PTPN22 1858T allele in Han people in Xiamen area．It is suggested that there's no association between PTPN22 C1858T polymorphism and RA．

Investigating the correlation between PTPN22 C1858T polymorphism and rheumatoid arthritis in Han nationality in Xiamen area

Objective To assess the correlation between FFPN22 C1858T polymorphism and rheumatoid arthritis(RA)in Han people in Xiamen area by TaqMan-MGB real-time PCR．Methods A casecontrol study was carried out in Xiamen Han population．Their blood samples(100 RAs and 100 controls respectively)were collected and the PTPN22 C1858T polymnrphism was tested by TaqMan-MGB real-time PCR．Results ①A technique of TaqMan-MGB teal-time PCR was established to investigate PTPN22 C1858T polymorphism；② Onlv 1858 C allele was presented in all the RAs and controls，and no T allele was detected．Conclusion There is no PTPN22 1858T allele in Han people in Xiamen area．It is suggested that there's no association between PTPN22 C1858T polymorphism and RA．