功能矫治前伸下颌后大鼠髁突软骨雌激素受体变化规律的研究

目的了解雌激素受体（ｅｓｔｒｏｇｅｎｒｅｃｅｐｔｏｒ，ＥＲ）与髁突软骨增生、分化及适应性生长改建的关系，探讨功能矫形治疗的机理。方法选用５０只４周龄的雌性ＳＤ大鼠，分为实验组和对照组。实验组大鼠配戴自制功能性矫治器引导下颌前伸，应用葡聚糖活性炭吸附法（ＤＣＣ法）对髁突软骨中ＥＲ进行了检测和分析。结果大鼠髁突软骨中存在雌激素受体，且髁突软骨增生旺盛期时受体含量较高，而分化期时含量较低；功能矫形前伸下颌后，髁突软骨中ＥＲ含量明显增加。结论雌激素受体与髁突软骨的增生、分化及功能矫形后的适应性生长改建关系密切。本项研究证实了髁突软骨中存在雌激素受体的作用机制并进一步阐明了功能矫形治疗的机理。

An experimental study on the changes of estrogen receptor in the condylar cartilages with DCC method in rats after functional mandibular protrusion

Objective The purpose of the study was to evaluate the estrogen receptors ( ER ) in the condylar cartilages in relation to the hyperplasia, hypertrophy and functional adaptive remodelling after functional mandibular protrusion. Methods Dextran coated charcoal ( DCC ) method was performed to quantitively detect the level of ER. Fifty female rats of 4 week old were selected and simulated functional appliances were used to guide their mandibles. The rats were sacrificed after 3 days, 1 week, 2 week, 3 week and 4 week. Results The results showed ER do exist in the condylar cartilages of the rats, with the highest levels during their actively proliferative period and the amount of ER was increased significantly after functional mandibular protrusion. Conclusion It is suggested that ER has a close relationship with the hyperplasia, hypertrophy of the condylar cartilages and plays a very important role in the adaptive remodelling mechanism after functional mandibular protrusion.