| 小鼠胚胎干细胞植入大鼠脑内分化的研究(英文) |
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| 引用本文： | 刘述,谢瑶,陈系古,姚志彬.小鼠胚胎干细胞植入大鼠脑内分化的研究(英文)[J].中国组织工程研究与临床康复,2003,7(16):2258-2259. |
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| 作者姓名： | 刘述 谢瑶 陈系古 姚志彬 |
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| 作者单位： | 1. 中山医科大学,解剖学教研室,广东省广州市,510080
2. 中山医科大学,实验动物中心,广东省广州市,510080 |
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| 基金项目： | 广东省重点攻关课题(203078)~~ |
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| 摘 要： | 目的观察小鼠胚胎干(ES)细胞植人大鼠隔区和海马内后的分化情况,并对其作研究和分析。方法成年SD大鼠35只,体质量200～250g,雌雄不限。以SD大鼠为宿主,将ES细胞移植入宿主隔区和海马内,移植后l,2,3,4和8周后取脑,冷冻切片,进行Nissl,M6,NSE,GFAP免疫组织化学染色。结果ES细胞移植入大鼠隔区和海马内后,从第2周开始表达M6、GFAP、NSE等抗原,持续至第4周,主要位于移植区内,较少迁移。结论小鼠ES细胞移植入大鼠隔区和海马内后,分化为神经元,神经胶质细胞。
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| 关 键 词： | 脑组织移植 细胞分化 免疫组织化学 |
Differentiation of mouse embryonic stem cells after transplantation into the adult rat brain |
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| Shu Liu,Yao Xie,Xi Gu Chen,Zhi BinYao.Differentiation of mouse embryonic stem cells after transplantation into the adult rat brain[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2003,7(16):2258-2259. |
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| Authors: | Shu Liu Yao Xie Xi Gu Chen Zhi BinYao |
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| Institution: | Shu Liu1,Yao Xie1,Xi Gu Chen2,Zhi BinYao1,1Department of Anatomy,2Department of Experimental Animal Centre,Sun Yat sen University of Medical Sciences,Guangzhou 510080,Guangdong Province,China |
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| Abstract: | Aim To observe differentiation of ES cells of mice in adult rat brain after transplantation into septum and hippocampus.Methods 35 adult SD rats weighed 200-250 g without limitation of sex were used.ES BALB/C cells were implanted into hippocampus and septum of adult rat,brain was taken at 1st,2nd,3th,4th,8th week.Sections were frozen and Nissl's staining,M6,NSE,GFAP immunohistochemical staining were performed.Results ES grafts expressed M6,GFAP,NSE antigen from second week after implantation into septum and hippocampus which were mainly located in implantation region.Conclusion Transplanted mice ES cells can differentiate into neurons, glia in the septum and hippocampus of rat brain. |
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| Keywords: | brain tissue transplantation cell differentiation immunohistochemistry |
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