兔准分子激光角膜切削术后角膜血小板源性生长因子mRNA表达的变化

研究角膜上皮下混浊形成的发生机制，检测准分子激光屈光性角膜切削术后角膜上皮和基质血小板源性生长因子表达的变化。方法新西兰白兔施行ＰＲＫ后１，２，３月用裂隙灯显微镜观察ｈａｘｅ形成情况，并用原位酸分子杂交方法，检测角膜上皮和基质ＰＤＧＦ ｍＲＮＡ的表达。结果正常角膜上皮细胞有ＰＤＧＦ ｍＲＮＡ表达，基质层无表达；ＰＲＫ后角膜上皮细胞ＰＤＧＦｍＲＮＡ表达增加，术后２月表达最强，且基质中亦有轻微表达。上

Expression of platelet-derived growth factor mRNA in rabbit cornea after photorefractive keratectomy

ObjectiveTo study the mechanism of haze formation and to investigate the expression of platelet derived growth factor (PDGF) mRNA in corneal epithelium and stroma after photorefractive keratectomy (PRK).MethodsTwenty white rabbits were randomly divided into 4 groups,and PRK was performed on each eye of 15 rabbits.Haze formation was determined by a slit lamp examination at the 1st,2nd and 3rd month after PRK,and the expression of PDGF mRNA was detected by in situ hybridization.ResultsThe corneal haze was first seen one month after PRK.The most obvious haze formation was observed at two months,and haze decreased or disappeared by the 3rd month after PRK.PDGF mRNA expression was present in the normal corneal epithelium,but was not present in the normal corneal stroma.Expression of PDGF mRNA in the corneal epithelium increased after PRK,with the peak appearing in the 2nd month.PDGF expression was detected in corneal stroma in the 2nd month.The extent of the expression of PDGF mRNA was related to the amount of haze formation.ConclusionPDGF takes part in promoting corneal wound healing after PRK,and may contribute to the formation and development of corneal haze.