血细胞处理仪的改进研究

目的：对血细胞处理仪（ BBS926型全自动医用低速离心机）处理冰冻红细胞技术进行改进研究，确定冰冻红细胞洗涤程序和效果。方法取源于400 ml （2 U）全血的健康献血者悬浮红细胞，-80℃冰冻保存，采用血细胞处理仪对解冻红细胞进行处理，在初始程序（程序1）洗涤冰冻红细胞的基础上，通过对洗液量和洗涤步骤等进行改进，形成新的优化程序（程序2）洗涤冰冻红细胞；对两组程序洗涤后的冰冻红细胞质量进行评价。结果两组洗涤程序（程序1和程序2）处理后的冰冻红细胞质量各项指标检测：血红蛋白（Hb）含量（g）分别为37．55±3．58和42．18±3．35（P＜0．05），游离血红蛋白（FHb）含量（g/L）分别为0．51±0．08和0．53±0．07（P＞0．05），白细胞残留量（×10^7个）分别为1．90±0．99和1．92±1．04（P＞0．05），晶体渗透压（mOsm）分别为334±8．03和327±9．06（P＞0．05），无菌实验普通细菌和真菌检测均为阴性。红细胞体外溶血率（％）分别为12．44±8．24和12．02±5．78（P＞0．05），红细胞变形性（EI）分别为21．40±1．41和21．42±1．45（P＞0．05），红细胞回收率（％）分别为72．02±3．70和77．18±5．58（P＜0．05），红细胞凋亡（％）分别为1．12±0．54和1．10±0．61（P＞0．05），洗涤时间（min）分别为79．00±0．71和79．60±0．55（P＞0．05）。结论两组洗涤程序洗涤冰冻红细胞质量均达到国家冰冻解冻去甘油红细胞质量控制要求，改进后洗涤程序2处理的冰冻红细胞Hb含量和红细胞回收率有一定提高；程序2洗涤效果优于程序1。

Protocols of improving red blood cells processing devices

Objective To improve the protocols of red blood cells （ RBCs） processing devices （ automatic medical RBC centrifuge, type：BBS926）.Methods RBCs separated from 400 ml of whole blood collected from healthy donors were frozen at -80℃.After thawing , the cells were processed by the washing device .Based on the original protocol （ protocol 1）, a modified protocol （protocol 2） was established and used to evaluate the quality of the frozen RBCs .In the test group （protocol 2）, the amount of washing buffers and the washing steps were revised to form the optimized protocol .RBCs processed with the two protocols were evaluated by different assays .Results The indexes from the standards for frozen-thawed RBCs： the amount of hemoglobin （ Hb） of RBCs from protocol 1 and protocol 2 was 37.55 ±3.58 and 42.18 ±3.35 g（P〈0.05）,respectively;the amount of free hemoglobin（FHb） was 0.51 ±0.08 g/L and 0.53 ±0.07 g/L （P〉0.05）;the residual amount of white blood cells （WBCs） was （1.90 ±0.99） ×10^7 and （1.92 ±1.04） ×10^7（P〉0.05）;The osmolarities were 334 ±8.03 mOsm and 327 ±9.06 mOsm（P〉0.05）;both the bacteria and fungi tests were negative for the RBCs processed with the two protocols .Among other indexes ,the hemolysis rate for RBCs from protocol 1 and protocol 2 was （12.44 ±8.24）%and （12.02 ±5.78）%（P〉0.05）, the deformation index was 21.40 ±1.41 and 21.42 ±1.45 （P〉0.05）, the RBC recovery was（72.02 ±3.70）%and （77.18 ±5.58）%（P〈0.05）,the cell apopto-sis rate was（1.12 ±0.54）%and （1.10 ±0.61）%（P〉0.05）,and the processing time was （79.00 ±0.71）min and （79.60 ±0.55）min （P〉0.05）.Conclusion The RBCs processed by the two protocols meet the national standards for frozen-thaw RBCs.Hb amounts and cell recoveries of the RBCs are enhanced by treatment with protocol 2.Protocol 2 proves to be better than protocol 1.