人工关节假体材料表面物理特性对结核分枝杆菌黏附的影响研究

目的：探讨不同人工关节假体材料表面物理特性对结核分枝杆菌（ MTB ）黏附的影响。方法以表面抛光涂层、钛涂层和羟基磷灰石涂层为实验材料，对材料进行表面形貌分析以及表面粗糙度、接触角和表面能的测定。选用MTB为研究菌株，体外共培养法培养，通过扫描电镜对MTB的形态、在材料表面的黏附情况和黏附量进行观察。综合评价人工关节假体材料的表面物理特性对细菌增殖和黏附的影响。结果钛涂层和羟基磷灰石涂层材料表面为疏水性材料，抛光涂层为亲水性材料，接触角及表面能存在显著性差异（P＜0．05）。细菌培养过程中3种材料菌液的D值无显著性差异（P＞0．05）。 MTB在钛涂层介面黏附量最多，羟基磷灰石涂层次之，抛光涂层介面最低，且各种材料间差异有统计学意义（P＜0．05）。结论 MTB在人工关节假体材料表面的黏附与其表面物理特性有关，在粗糙度高及疏水性低的材料表面有较高的黏附性。

Impact of surface physical properties of artificial joint prosthesis materials on adhesion of Mycobacterium tuberculosis

Objective To study the effect of surface physical properties of different materials for the artificial joint pros -thesis interface on Mycobacterium tuberculosis adhesion .Methods The surface polishing coating , titanium coating and hydroxyapatite coating were chosen as the experimental materials to analyze the surface topography and measure the surface roughness, contact angle and surface energy .The M.tuberculosis strains were used by in vitro cultivation method and ob-served by scanning electron microscopy （SEM） to study the morphology of M.tuberculosis and the material surface adhe-sion.The influence of surface physical properties of the interface of the artificial joint prostheses on the proliferation and ad -hesion of bacteria was evaluatd .Results The titanium coating and hydroxyapatite coating materials were hydrophobic , while the surface polishing coating was hydrophilic .The contact angle and surface energy were significantly different （ P〈0.05）.In the process of bacterial culture of the three types of materials , there was no significant difference （P〉0.05）in the OD value of bacterial solution .The titanium coating had the largest amount of interface adhesion for M.tuberculosis, fol-lowed by the hydroxyapatite coating and the polishing coating .The difference between various materials was statistically sig-nificant （P〈0.05）.Conclusion The adhesion of M.tuberculosis in the surface of artificial joint prosthesis materials is closely related to the surface physical properties .A adhesiveness improves with surface roughness .