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| 胱硫醚-β-合成酶在年轻和老年大鼠海马中的表达分布 | |
| 引用本文： | 刘磊,乔伟丽,祁友键,徐红,闫长栋.胱硫醚-β-合成酶在年轻和老年大鼠海马中的表达分布[J].徐州医学院学报,2010,30(10):645-647. |
| 作者姓名： | 刘磊 乔伟丽 祁友键 徐红 闫长栋 |
| 作者单位： | 1. 徐州医学院生理学教研室,江苏,徐州,221002;徐州医学院神经生物学教研室 2. 徐州医学院生理学教研室,江苏,徐州,221002 3. 徐州医学院神经生物学教研室 |
| 基金项目： | 江苏省自然科学基金，江苏省普通高校研究生创新计划 |
| 摘 要： | 目的探讨硫化氢（H2S）合成酶胱硫醚-β-合成酶（CBS）在海马中的分布和表达是否受年龄因素的影响。方法用免疫组化和免疫印迹方法观察年轻（3月龄）和老年（24月龄）大鼠海马中CBS的分布和含量。结果 CBS在年轻和老年大鼠海马中主要分布在颗粒细胞层和锥体细胞层,呈区域性分布,其中CA1区表达最多,CA3区、DG区表达较少;CBS在老年大鼠和年轻大鼠海马中的表达和分布没有变化。结论 CBS在大鼠海马中呈区域性分布,CA1区表达最多,年龄不影响CBS的分布和表达。 |
| 关 键 词： | 硫化氢 胱硫醚-β-合成酶 海马 |
Expression distribution of cystathionine-β-synthase in hippocampus of young and old rats |
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| LIU Lei,QIAO Weili,QI Youjian,XU Hong,YAN Changdong.Expression distribution of cystathionine-β-synthase in hippocampus of young and old rats[J].Acta Academiae Medicinae Xuzhou,2010,30(10):645-647. | |
| Authors: | LIU Lei QIAO Weili QI Youjian XU Hong YAN Changdong |
| Institution: | 1.Department of Physiology,Xuzhou Medical College,Xuzhou,Jiangsu 221002,China;2.Department of Neurobiology,Xuzhou Medical College) |
| Abstract: | Objective To explore the correlation between the distribution and expression of hydrogen sulfide（H2S） synthase cystathionine-β-synthase（CBS） in the hippocampus of young（3 months） and aged（24 months） rats.Methods The distribution and expression of CBS in the hippocampus of young and aged rats were determined by immunohistochemistry and Western blot analysis.Result The expression of CBS showed a regional distribution in the hippocampus of young and old rats,in which CBS was highly expressed in CA1 area,and much less expressed in CA3 area and DG area.Compared with young rats,there was no variation in the expression of CBS in hippocampus of aged rats.Conclusion The expression of CBS showed a regional distribution in the hippocampus of young and old rats and had the top level of expression in CA1 region,with age factors unrelated in the distribution and expression of CBS. |
| Keywords: | hydrogen sulfide cystathionine-β-synthase hippocampus |
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