干燥综合征患者外周血中T淋巴细胞亚群的分析与临床意义

目的探讨干燥综合征(SS)患者外周血T淋巴细胞亚群的变化及其临床意义。方法用流式细胞仪检测并比较SS患者与正常对照者外周血T淋巴细胞亚群有无差异。以红细胞沉降率及血清IgG来判断SS病情活动度,分析SS患者中T淋巴细胞亚群与病情活动的关系。结果与正常对照组相比,SS患者外周血中CD8+T淋巴细胞、CD8+CD28+T淋巴细胞构成比及CD8+/CD4+比值均显著降低;而CD4+T淋巴细胞、CD4+CD45RA+T淋巴细胞及CD4+CD28+T淋巴细胞在两组中则差异无统计学意义。与病情非活动者相比,病情活动的SS患者的CD8+T淋巴细胞构成比及CD8+/CD4+比值均显著降低。结论SS患者外周血T淋巴细胞亚群存在异常,并与SS的病情活动有关,可能在SS发病中具有重要作用。

Clinical significance of T lymphocyte subsets in peripheral blood from patients with Sj(o)gren syndrome

Objective To investigate the clinical significance of T lymphocyte subsets in peripheral blood from patients with Sjogren Syndrome(SS).Methods Lymphocyte subsets were determined in the peripheral blood from SS patients and healthy controls with two-color flow cytometry using various monoclonal antibodies.The relationship between T lymphocyte subsets and disease activity as measured by erythrocyte sedimentation rate(ESR) and serum IgG were analyzed.Results Percentages of CD8 ,CD8 CD28 T lymphocyte subsets and the ratio of CD8 / CD4 were significantly decreased in SS patients as compared with controls(P<0.05),while percentages of CD4 ,CD4 CD45RA and CD4 CD28 T lymphocyte subsets did not differ between controls and SS(P>0.05).Percentage of CD8 T lymphocyte subsets and the ratio of CD8 /CD4 were significantly decreased in patients with active SS.Conclusion Abnormal T lymphocyte subsets were seen in peripheral blood from SS patients and related to the disease activity of SS,which could play an important role in the pathogenesis of SS.