雌激素受体基因多态性与绝经后干眼症的相关性的研究

目的:研究雌激素受体(estrogen receptor,ER)基因多态性与绝经后干眼症的相关性。方法:收集绝经后干眼症患者65例及正常对照73例血液标本,提取基因组DNA,用聚合酶链反应方法检测ER基因的XbaⅠ和PvuⅡ酶切多态性并进行统计学分析。结果:干眼症患者与正常人比较,ER基因的PvuⅡ酶切多态性存在显著性差异(P<0.05),而ER基因的XbaⅠ酶切多态性没有明显差异(P>0.05)。结论:ER基因的PvuⅡ酶切多态性与绝经后干眼症发病存在相关性,不同个体的基因差异可能影响干眼症的发生。

The Correlation of Polymorphism of Estrogen Receptor Gene to Dry Eye Syndrome in Postmenopausal Women

PURPOSE: To investigate the correlation of estrogen receptor (ER) gene polymorphism to dry eye syndrome in postmenopausal women. METHODS: Xba I and Pvu II polymorphism of ER gene were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 65 patients with dry eye syndrome and 73 with controls in postmenopausal women. RESULTS: There was a significant difference of Pvu II polymorphism of ER gene between the patients of dry eye syndrome and controls in postmenopausal women (P < 0.05), but no significant difference was found in Xba I polymorphism of ER gene (P > 0.05). CONCLUSION: There is a significant correlation of Pvu II polymorphism of ER gene with dry eye syndrome in postmenopausal women. It is indicated that the difference in ER genotypes might influence the development of dry eye syndrome.