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镰形棘豆总黄酮提取工艺的比较研究
引用本文:郭敏,王晓琳,黄聪琳,姜华.镰形棘豆总黄酮提取工艺的比较研究[J].甘肃中医,2014,0(2):34-35.
作者姓名:郭敏  王晓琳  黄聪琳  姜华
作者单位:郭敏 (甘肃省中医院,甘肃 兰州730050 甘肃省中医药研究院); 王晓琳 (甘肃省中医院,甘肃 兰州730050 甘肃省中医药研究院); 黄聪琳 (甘肃省中医院,甘肃 兰州730050 甘肃省中医药研究院); 姜华 (甘肃省中医药研究院);
基金项目:国家自然科学基金项目(项目编号:编号81260633)
摘    要:目的:比较4种不同工艺对镰形棘豆总黄酮提取的影响。方法:采用水提醇沉酸解、水提、碱提酸沉及水提酸解4种工艺提取,以镰形棘豆总黄酮得率及纯度为指标,NaNO2-Al(NO3)3比色法测定镰形棘豆总黄酮含量。结果:镰形棘豆最佳提取工艺为水提酸解。该提取物中总黄酮提取率为1.6%,纯度为:11.26%。结论:该工艺可用于镰形棘豆药材中总黄酮成分的提取,方法可靠,简便易行。

关 键 词:镰形棘豆  总黄酮  提取工艺

Comparative Study on the Extraction Processes of Total Flavonoids from LianXing JiDou
GUO Min,WANG Xiaolin,HUANG Conglin,JIANG Hua.Comparative Study on the Extraction Processes of Total Flavonoids from LianXing JiDou[J].Gansu Journal of Traditional Chinese Medicine,2014,0(2):34-35.
Authors:GUO Min  WANG Xiaolin  HUANG Conglin  JIANG Hua
Institution:1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Gansu Provincial Academy of Chinese Medicine)
Abstract:Objective:To compare the effects of four kinds of processes on the extraction of total flavonoids from LianXing JiDou(Oxytropis falcata Bunge). Methods:The contents of total flavonoids from LianXing JiDou were detected by NaNO2-Al(NO3) 3 chromatometry, the yield and the purity of total flavonoids were taken as the indexes, total flavonoids were extracted with the processes including water-extraction alcohol-precipitation and acid-hydroly-sis, water-extraction, alkaline-extraction and acid-precipitation, water-extraction and acid-hydrolysis. Results: The best extraction process for LianXing JiDou was water-extraction and acid-hydrolysis. The extraction percentage and the purity of total flavonoids were 1.6%and 11.26%respectively. Conclusion:The process could be used to extract total flavonoids from LianXing JiDou, which is reliable, simple and practicable.
Keywords:LianXing JiDou  total flavonoids  extraction process
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