| 黑热病快速ELISA诊断方法的研究 |
| |
| 引用本文： | 王秀珍 黎世涛. 黑热病快速ELISA诊断方法的研究[J]. 地方病通报, 1990, 5(2): 48-51 |
| |
| 作者姓名： | 王秀珍 黎世涛 |
| |
| 作者单位： | 四川省医学科学院寄生虫病防治研究所 成都(王秀珍，黎世涛，尹治成)，四川省医学科学院寄生虫病防治研究所 成都(周肇西) |
| |
| 摘 要： |
|
| 关 键 词： | 黑热病 单克隆抗体 ELISA 诊断 |
Development and Application of an ELISA Kit Containing Anti-human Gamma Chain McAb for Rapid Diagnosis of Leishmaniasis |
| |
| Wang Xiu-zhen,Li Shi-tao,Yin Zhi-cheng,Zhou Zhao-xi. Development and Application of an ELISA Kit Containing Anti-human Gamma Chain McAb for Rapid Diagnosis of Leishmaniasis[J]. Endemic Diseases Bulletin, 1990, 5(2): 48-51 |
| |
| Authors: | Wang Xiu-zhen Li Shi-tao Yin Zhi-cheng Zhou Zhao-xi |
| |
| Abstract: | A kit consisted of PVC film prepared by coating antigen from cultured promastigotes in RPMI 1640, enzyme conjugate of anti-human gamma chain McAb and TMB substrate, was used for rapid diagnosis of leishmaniasis and the results were compared with those by routine ELFSA. Sera from 37 cases of leishmeniasis identified by bone marrow smear examinationwere tested with a result of 100% positive reaction rate. No false positive reaction was observed among 120 serum samples from normal individuals. Cross reactions were not found between leishmaniasis and schistosomiasis, vivax malaria, cysticercosis, trichinosis as well as clonolchiasis, except leprosy which had a 10% positive reaction rate among 30 cases. The results showed that the ELISA kit for rapid diagnosis of leishmaniasis has high sensitivity, good specificity and can be compared with the routine one but is much easier and quicker to perform. |
| |
| Keywords: | Visceral leishmaniasis Diagnosis McAb ELISA |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
