Immunosuppression in hamsters with progressive visceral leishmaniasis: an evaluation of the role of nitric oxide toward impairment of the lymphoproliferative response |
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Authors: | Subhajit Dasgupta Ananda Mookerjee Sanjib K Chowdhury Asoke C Ghose |
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Institution: | (1) Department of Microbiology, Bose Institute, Calcutta 700 054, India, IN;(2) Organon Research Center, Calcutta 700 016, India, IN;(3) Department of Microbiology, Bose Institute, P-1/12 CIT Scheme VII M, Calcutta 700 054, India E-mail: acghosh@boseinst.ernet.in, Fax: +91-33-334-3886 , IN |
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Abstract: | The progressive visceral infection caused in golden hamsters by Leishmania donovani amastigotes led to gradual impairment of the proliferative response of their splenic (SPMC) or peripheral blood (PBMC) mononuclear
cells to in vitro stimulation with leishmanial antigen, with mitogen (concanavalin A), and even with a combination of phorbol
myristate acetate (PMA) and ionomycin (Io). Removal of macrophage-like adherent cells from SPMC or PBMC of infected animals,
however, almost completely restored their proliferative response to PMA + Io, thus ruling out the possibility of any intrinsic
defect in the signal-transduction pathways of lymphocyte activation and proliferation. Subsequent studies demonstrated that
the generation of soluble mediators such as nitric oxide by these adherent cells is responsible, albeit partially, for the
down-regulation of the lymphoproliferative response in hamsters with visceral leishmaniasis.
Received: 8 December 1998 / Accepted: 15 January 1999 |
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